Dr. Wolfgang H. Becker Profile

Dr. Wolfgang H. Becker

President at Becker & Hickl GmbH

SPIE Involvement:

Conference Program Committee | Author

Publications (63)

SPIE Journal Paper | 30 September 2024 Open Access

Open Access

Simultaneous assessment of NAD(P)H and flavins with multispectral fluorescence lifetime imaging microscopy at a single excitation wavelength of 750 nm

Boris Yakimov, Anastasia Komarova, Elena Nikonova, Artem Mozherov, Liubov Shimolina, Marina Shirmanova, Wolfgang Becker, Evgeny Shirshin, Vladislav Shcheslavskiy

JBO, Vol. 29, Issue 10, 106501, (September 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.10.106501

KEYWORDS: Fluorescence, Fluorescence lifetime imaging, Nicotinamide adenine dinucleotide, Emission wavelengths, Mode conditioning cables, Sensors, Fluorescence intensity, Cancer, Microscopy, Tumors

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SignificanceAutofluorescence characteristics of the reduced nicotinamide adenine dinucleotide and oxidized flavin cofactors are important for the evaluation of the metabolic status of the cells. The approaches that involve a detailed analysis of both spectral and time characteristics of the autofluorescence signals may provide additional insights into the biochemical processes in the cells and biological tissues and facilitate the transition of spectral fluorescence lifetime imaging into clinical applications.AimWe present the experiments on multispectral fluorescence lifetime imaging with a detailed analysis of the fluorescence decays and spectral profiles of the reduced nicotinamide adenine dinucleotide and oxidized flavin under a single excitation wavelength aimed at understanding whether the use of multispectral detection is helpful for metabolic imaging of cancer cells.ApproachWe use two-photon spectral fluorescence lifetime imaging microscopy. Starting from model solutions, we switched to cell cultures treated by metabolic inhibitors and then studied the metabolism of cells within tumor spheroids.ResultsThe use of a multispectral detector in combination with an excitation at a single wavelength of 750 nm allows the identification of fluorescence signals from three components: free and bound NAD(P)H, and flavins based on the global fitting procedure. Multispectral data make it possible to assess not only the lifetime but also the spectral shifts of emission of flavins caused by chemical perturbations. Altogether, the informative parameters of the developed approach are the ratio of free and bound NAD(P)H amplitudes, the decay time of bound NAD(P)H, the amplitude of flavin fluorescence signal, the fluorescence decay time of flavins, and the spectral shift of the emission signal of flavins. Hence, with multispectral fluorescence lifetime imaging, we get five independent parameters, of which three are related to flavins.ConclusionsThe approach to probe the metabolic state of cells in culture and spheroids using excitation at a single wavelength of 750 nm and a fluorescence time-resolved spectral detection with the consequent global analysis of the data not only simplifies image acquisition protocol but also allows to disentangle the impacts of free and bound NAD(P)H, and flavin components evaluate changes in their fluorescence parameters (emission spectra and fluorescence lifetime) upon treating cells with metabolic inhibitors and sense metabolic heterogeneity within 3D tumor spheroids.

Proceedings Article | 13 March 2024 Presentation

Presentation

A novel integration of 1P confocal- and multiphoton-FLIM in one system

Taravat Saeb Gilani, Lukas Braun, Julius Heitz, Hauke Studier, Wolfgang Becker

Proceedings Volume PC12847, PC1284711 (2024) https://doi.org/10.1117/12.3005810

KEYWORDS: Fluorescence lifetime imaging, Second harmonic generation, Tissues, Imaging systems, Biological imaging, Laser stabilization, Confocal microscopy, Cancer detection, Visualization, Tunable lasers

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Proceedings Article | 13 March 2024 Presentation

Presentation

Ever faster FLIM: why speed and sensitivity are no contradiction (anymore)

Julius Heitz, Lukas Braun, Taravat Saeb-Gilani, Hauke Studier, Wolfgang Becker

Proceedings Volume PC12847, PC128470U (2024) https://doi.org/10.1117/12.3005144

KEYWORDS: Fluorescence lifetime imaging, Video, Video processing, In vivo imaging, Imaging systems, Biological samples

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Proceedings Article | 13 March 2024 Presentation

Presentation

Deep insights from 3D FLIM: metabolic FLIM analysis taken to the 3rd dimension

Lukas Braun, Julius Heitz, Taravat Saeb-Gilani, Hauke Studier, Wolfgang Becker

Proceedings Volume PC12847, PC128470X (2024) https://doi.org/10.1117/12.3005165

KEYWORDS: Fluorescence lifetime imaging, 3D image processing, 3D metrology, Data analysis, Confocal microscopy, Cancer detection, Cancer, Autofluorescence

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Proceedings Article | 25 April 2023 Presentation + Paper

Presentation + Paper

Ultra-fast fluorescence decay in biological material

Lukas Braun, Julius Heitz, Vladislav Shcheslavskiy, Cornelia Junghans, Oksana Garanina, Vadim Elagin, Wolfgang Becker

Proceedings Volume 12384, 1238406 (2023) https://doi.org/10.1117/12.2655628

KEYWORDS: Picosecond phenomena, Fluorescence lifetime imaging, Tissues, Ultrafast phenomena, Fluorescence, Melanoma, Time correlated single photon counting, Histograms, Skin, Materials processing

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Proceedings Article | 15 March 2023 Presentation

Presentation

Endoscopic multiphoton fluorescence lifetime imaging of NADH (Conference Presentation)

Julius Heitz, Lukas Braun, Dennis Eggert, Hauke Studier, Wolfgang Becker, Christian Betz

Proceedings Volume PC12384, PC123840W (2023) https://doi.org/10.1117/12.2666398

KEYWORDS: Nicotinamide adenine dinucleotide, Fluorescence lifetime imaging, Endoscopy, Multiphoton fluorescence microscopy, Tissues, Autofluorescence, Mode conditioning cables, Endoscopes, Time correlated single photon counting, Technology

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Proceedings Article | 5 March 2021 Presentation

Presentation

Functional imaging progress for clinical use

Hauke Studier, Amy Holmes, Michael Pastore, Wolfgang Becker, Michael Roberts

Proceedings Volume 11648, 116480F (2021) https://doi.org/10.1117/12.2578231

KEYWORDS: Fluorescence lifetime imaging, Functional imaging, Melanoma, Tissues, Skin, Safety, Nanoparticles, In vivo imaging, Diagnostics, Data analysis

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Proceedings Article | 5 March 2021 Presentation

Presentation

Getting more from TCSPC FLIM data: SPCImage NG next generation data analysis software

Wolfgang Becker, Vladislav Shcheslavskiy, Axel Bergmann

Proceedings Volume 11648, 1164809 (2021) https://doi.org/10.1117/12.2578326

KEYWORDS: Fluorescence lifetime imaging, Data analysis, Tissues, Modeling, Image segmentation, Image analysis, Graphics processing units, Data processing

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Proceedings Article | 5 March 2021 Presentation

Presentation

Metabolic FLIM at the dawn of clinical application

Wolfgang Becker, Lukas Braun

Proceedings Volume 11648, 116480J (2021) https://doi.org/10.1117/12.2578319

KEYWORDS: Fluorescence lifetime imaging, Tumors, Luminescence

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Proceedings Article | 9 March 2020 Presentation

Presentation

Multiphoton FLIM is gaining ground as a clinical tool (Conference Presentation)

Hauke Studier, Yousuf Mohammed, Michael Roberts, Amy Holmes, Michael Pastore, Wolfgang Becker

Proceedings Volume 11244, 112441Q (2020) https://doi.org/10.1117/12.2544795

KEYWORDS: Fluorescence lifetime imaging, Nanoparticles, Skin, Melanoma, Toxicity, Multiphoton fluorescence microscopy, Skin cancer, Life sciences, Safety, Sun

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Proceedings Article | 14 February 2020 Presentation + Paper

Presentation + Paper

Metabolic imaging by simultaneous 2-photon FLIM of NAD(P)H and FAD

Wolfgang Becker, Axel Bergmann, Alexander Jelzow, Antje Neubauer, Angelika Rück, Konrad Birkmeier, Patrick Leisching

Proceedings Volume 11244, 112440L (2020) https://doi.org/10.1117/12.2550962

KEYWORDS: Fluorescence lifetime imaging, Multiplexing, Second-harmonic generation, Sensors, Photons, Imaging systems, Picosecond phenomena, Femtosecond fiber lasers, Luminescence, Ultrafast phenomena

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Proceedings Article | 4 March 2019 Presentation

Presentation

Ophthalmic FLIM for eye disease studies: advanced technical solutions (Conference Presentation)

Cornelia Junghans, Hauke Studier, Wolfgang Becker

Proceedings Volume 10882, 108820X (2019) https://doi.org/10.1117/12.2509872

KEYWORDS: Fluorescence lifetime imaging, Eye, Tissues, Luminescence, Ophthalmic imaging, Spectral resolution, Retinal scanning, Ophthalmoscopy, Proteins, Ophthalmology

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Proceedings Article | 4 March 2019 Presentation

Presentation

Skin research with multiphoton FLIM (Conference Presentation)

Hauke Studier, Wolfgang Becker, Michael Roberts, Michael Pastore, Amy Holmes

Proceedings Volume 10882, 108820L (2019) https://doi.org/10.1117/12.2508665

KEYWORDS: Skin, Fluorescence lifetime imaging, Melanoma, Skin cancer, Multiphoton fluorescence microscopy, Nanoparticles, Toxicity, Tissue optics, Surgery, Safety

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Proceedings Article | 22 February 2019 Presentation + Paper

Presentation + Paper

Interrogation of glioma metabolism on macroscale by FLIM

Marina Shirmanova, Maria Lukina, Elena Kisileva, Vera Fedoseeva, Varvara Dudenkova, Elena Zagaynova, Wolfgang Becker, Vladislav Shcheslavskiy

Proceedings Volume 10882, 1088209 (2019) https://doi.org/10.1117/12.2511475

KEYWORDS: Tumors, Brain, Luminescence, Fluorescence lifetime imaging, Tissues, Mode conditioning cables, Picosecond phenomena, Animal model studies, Cancer, Medical research

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Proceedings Article | 22 February 2019 Presentation + Paper

Presentation + Paper

Fast-acquisition TCSPC FLIM with sub-25-ps IRF width

Wolfgang Becker, Axel Bergmann, Stefan Smietana

Proceedings Volume 10882, 1088206 (2019) https://doi.org/10.1117/12.2510272

KEYWORDS: Fluorescence lifetime imaging, Picosecond phenomena, Data acquisition, Photodetectors, Luminescence

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Proceedings Article | 22 February 2019 Presentation + Paper

Presentation + Paper

Metabolic imaging by simultaneous FLIM of NAD(P)H and FAD

Wolfgang Becker, Axel Bergmann, Rodrigo Suarez Ibarrola, Philippe-Fabian Müller, Lukas Braun

Proceedings Volume 10882, 108820B (2019) https://doi.org/10.1117/12.2510132

KEYWORDS: Confocal laser scanning microscopy, Clinical research, Fluorescence lifetime imaging, Luminescence, Tumors, Multiplexing, Signal detection, Data analysis, Cancer, Tissues

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Proceedings Article | 14 March 2018 Presentation

Presentation

Non-invasive metabolic imaging of melanoma progression (Conference Presentation)

Hauke Studier, Michael Roberts, Michael Pastore, Wolfgang Becker

Proceedings Volume 10498, 104980D (2018) https://doi.org/10.1117/12.2286280

KEYWORDS: Melanoma, Fluorescence lifetime imaging, Tumors, Mouse models, Mode conditioning cables, Multiphoton microscopy, Luminescence, Cancer, Skin cancer, Skin

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Proceedings Article | 14 March 2018 Presentation

Presentation

Macroscopic time-resolved imaging of tumor (Conference Presentation)

Vladislav Shcheslavskiy, Marina Shirmanova, Victoria Zherdeva, Alena Gavrina, Varvara Dudenkova, Elena Zagaynova, Alexander Savitskiy, Wolfgang Becker

Proceedings Volume 10498, 104980V (2018) https://doi.org/10.1117/12.2299317

KEYWORDS: Tumors, Scanners, Visualization, Proteins, Fluorescent proteins, In vivo imaging

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Proceedings Article | 23 February 2018 Presentation + Paper

Presentation + Paper

Ultra-fast HPM detectors improve NAD(P)H FLIM

Wolfgang Becker, Cornelia Wetzker

Proceedings Volume 10498, 1049806 (2018) https://doi.org/10.1117/12.2296544

KEYWORDS: Sensors, Fluorescence lifetime imaging, Picosecond phenomena, Signal to noise ratio, Ultrafast phenomena, Luminescence, Data modeling, Data analysis, Mode conditioning cables, Tumors

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Proceedings Article | 28 July 2017 Paper

Paper

Fiber-based time-resolved fluorescence and phosphorescence spectroscopy of tumors

M. Shirmanova, M. Lukina, A. Orlova, H. Studier, E. Zagaynova, W. Becker, V. Shcheslavskiy

Proceedings Volume 10413, 104130I (2017) https://doi.org/10.1117/12.2284185

KEYWORDS: Time resolved spectroscopy, Phosphorescence, Tumors, Fluorescence spectroscopy, Spectroscopy, Single photon, Fluorescence lifetime imaging, Luminescence, Fiber optics

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Proceedings Article | 21 February 2017 Presentation + Paper

Presentation + Paper

Online-FLIM at 10 images per second

Wolfgang Becker, Stefan Smietana

Proceedings Volume 10069, 1006916 (2017) https://doi.org/10.1117/12.2254725

KEYWORDS: Fluorescence lifetime imaging, Laser scanners, Luminescence, Image processing, Multiphoton microscopy, Data acquisition, Confocal microscopy, Scanners, Microscopy, Signal to noise ratio, Picosecond phenomena, Time correlated photon counting

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Proceedings Article | 5 May 2016 Paper

Paper

Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

Klaus Suhling, Liisa Hirvonen, Wolfgang Becker, Stefan Smietana, Holger Netz, James Milnes, Thomas Conneely, Alix Le Marois, Ottmar Jagutzki

Proceedings Volume 9858, 98580J (2016) https://doi.org/10.1117/12.2227198

KEYWORDS: Single photon, Microscopy, Fluorescence lifetime imaging, Sensors, Luminescence, Cameras, Fluorescence lifetime imaging, Microscopy, Microchannel plates, Picosecond phenomena, Time correlated photon counting, Phosphorescence

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Proceedings Article | 28 April 2016 Presentation

Presentation

Imaging of calcium transients in cultured neurons by TCSPC FLIM (Conference Presentation)

Wolfgang Becker, Samuel Frere

Proceedings Volume 9712, 97120O (2016) https://doi.org/10.1117/12.2214083

KEYWORDS: Fluorescence lifetime imaging, Calcium, Neurons, Single photon, Luminescence, Fluorescence spectroscopy, Photodetectors, Signal to noise ratio, Scanners, Multiphoton microscopy

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Proceedings Article | 27 April 2016 Presentation

Presentation

TCSPC FLIM in the wavelength range from 800 nm to 1700 nm (Conference Presentation)

Wolfgang Becker, Vladislav Shcheslavsky

Proceedings Volume 9723, 97230K (2016) https://doi.org/10.1117/12.2217500

KEYWORDS: Fluorescence lifetime imaging, Tissues, Luminescence, Near infrared, Sensors, Laser scanners, Picosecond phenomena, Optical microscopy, Signal detection, Multiphoton microscopy

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Proceedings Article | 28 February 2014 Paper

Paper

Megapixel FLIM

Hauke Studier, Klaus Weisshart, Oliver Holub, Wolfgang Becker

Proceedings Volume 8948, 89481K (2014) https://doi.org/10.1117/12.2039454

KEYWORDS: Fluorescence lifetime imaging, Microscopes, Luminescence, Data acquisition, Electronics, Confocal microscopy, Picosecond phenomena, Sensors, Data analysis, Laser scanners

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Proceedings Article | 22 February 2013 Paper

Paper

Fluorescence lifetime imaging with near-infrared dyes

Wolfgang Becker, Vladislav Shcheslavskiy

Proceedings Volume 8588, 85880R (2013) https://doi.org/10.1117/12.2003608

KEYWORDS: Fluorescence lifetime imaging, Near infrared, Luminescence, Beam splitters, Tissues, Confocal microscopy, Microscopes, Sensors, Picosecond phenomena, Skin

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Proceedings Article | 9 February 2012 Paper

Paper

Spatially resolved recording of transient fluorescence-lifetime effects by line-scanning TCSPC

Wolfgang Becker, Bertram Su, Axel Bergmann

Proceedings Volume 8226, 82260C (2012) https://doi.org/10.1117/12.908663

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Clocks, Picosecond phenomena, Scanners, Pulsed laser operation, Laser scanners, Microscopes, Photodetectors, Line scan image sensors

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Proceedings Article | 11 February 2011 Paper

Paper

Simultaneous fluorescence and phosphorescence lifetime imaging

Wolfgang Becker, Bertram Su, Axel Bergmann, Klaus Weisshart, Oliver Holub

Proceedings Volume 7903, 790320 (2011) https://doi.org/10.1117/12.875204

KEYWORDS: Phosphorescence, Luminescence, Pulsed laser operation, Modulation, Laser scanners, Microscopes, Clocks, Semiconductor lasers, Sensors, Confocal microscopy

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Proceedings Article | 26 February 2010 Paper

Paper

Better FLIM and FCS data by GaAsP hybrid detectors

Wolfgang Becker, Bertram Su, Axel Bergmann

Proceedings Volume 7569, 75690S (2010) https://doi.org/10.1117/12.843649

KEYWORDS: Fluorescence lifetime imaging, Sensors, Fluorescence correlation spectroscopy, Luminescence, Avalanche photodiodes, Signal detection, Picosecond phenomena, Signal to noise ratio, Digital image correlation, Microscopes

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Proceedings Article | 8 July 2009 Paper

Paper

Clinical results of fluorescence lifetime imaging in ophthalmology

D. Schweitzer, S. Quick, M. Klemm, M. Hammer, S. Jentsch, J. Dawczynski, W. Becker

Proceedings Volume 7368, 736802 (2009) https://doi.org/10.1117/12.831629

KEYWORDS: Picosecond phenomena, Luminescence, Ophthalmology, Laser scanners, Eye, Retina, Fluorescence spectroscopy, Mode conditioning cables, Crystals, Fluorescence lifetime imaging

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Proceedings Article | 25 February 2009 Paper

Paper

Simultaneous monitoring of the two coupled motors of a single FoF1-ATP synthase by three-color FRET using duty cycle-optimized triple-ALEX

N. Zarrabi, S. Ernst, M. Düser, A. Golovina-Leiker, W. Becker, R. Erdmann, S. Dunn, M. Börsch

Proceedings Volume 7185, 718505 (2009) https://doi.org/10.1117/12.809610

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Luminescence, Pulsed laser operation, Picosecond phenomena, Proteins, Confocal microscopy, Avalanche photodetectors, Bragg cells, Optical filters

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Proceedings Article | 13 February 2009 Paper

Paper

Fast-acquisition multispectral FLIM by parallel TCSPC

Wolfgang Becker, Bertram Su, Axel Bergmann

Proceedings Volume 7183, 718305 (2009) https://doi.org/10.1117/12.807697

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Picosecond phenomena, Confocal microscopy, Sensors, Electronics, Scanners, Fluorescence resonance energy transfer, Tissue optics, Data acquisition

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Proceedings Article | 15 February 2008 Paper

Paper

Multidimensional fluorescence lifetime measurements

Christoph Biskup, Birgit Hoffmann, Laimonas Kelbauskas, Thomas Zimmer, Nikolaj Klöcker, Wolfgang Becker, Klaus Benndorf

Proceedings Volume 6860, 68601I (2008) https://doi.org/10.1117/12.762834

KEYWORDS: Luminescence, Fluorescence resonance energy transfer, Proteins, Molecules, Microscopes, Confocal microscopy, Streak cameras, Sensors, Resonance energy transfer, Fluorescent proteins

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Proceedings Article | 15 October 2007 Paper

Paper

How fast can TCSPC FLIM be made?

Vicky Katsoulidou, Axel Bergmann, Wolfgang Becker

Proceedings Volume 6771, 67710B (2007) https://doi.org/10.1117/12.735550

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Sensors, Picosecond phenomena, Microscopes, Time correlated photon counting, Signal detection, Signal attenuation, Tissues, Photodetectors

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Proceedings Article | 11 October 2007 Open Access

Open Access

Paper

Multi-dimensional time-correlated single-photon counting

Wolfgang Becker, Axel Bergmann

Proceedings Volume 6771, 677102 (2007) https://doi.org/10.1117/12.735546

KEYWORDS: Photons, Luminescence, Sensors, Signal detection, Picosecond phenomena, Time metrology, Single photon, Clocks, Biomedical optics, Electronics

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Proceedings Article | 10 February 2007 Paper

Paper

Multidimensional fluorescence lifetime measurements

Christoph Biskup, Birgit Hoffmann, Laimonas Kelbauskas, Thomas Zimmer, Sascha Dietrich, Wolfgang Becker, Axel Bergmann, Nikolaj Klöcker, Klaus Benndorf

Proceedings Volume 6442, 64420V (2007) https://doi.org/10.1117/12.699870

KEYWORDS: Luminescence, Fluorescence resonance energy transfer, Molecules, Proteins, Microscopes, Streak cameras, Confocal microscopy, Sensors, Pulsed laser operation, Resonance energy transfer

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Proceedings Article | 3 November 2006 Open Access

Open Access

Paper

Multidimensional time-correlated single photon counting

Wolfgang Becker, Axel Bergmann

Proceedings Volume 6372, 637201 (2006) https://doi.org/10.1117/12.684742

KEYWORDS: Photons, Luminescence, Signal detection, Sensors, Single photon, Time metrology, Microscopes, Picosecond phenomena, Tissue optics, Fluorescence correlation spectroscopy

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Proceedings Article | 27 October 2006 Paper

Paper

Advanced time-correlated single photon counting technique for spectroscopy and imaging of biological systems

Wolfgang Becker, Axel Bergmann, Michal Kacprzak, Adam Liebert

Proceedings Volume 6047, 604714 (2006) https://doi.org/10.1117/12.710872

KEYWORDS: Luminescence, Tissue optics, Imaging spectroscopy, Imaging systems, Sensors, Picosecond phenomena, Single photon, Spectroscopy, Biomedical optics, Fluorescence resonance energy transfer

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Proceedings Article | 25 October 2006 Paper

Paper

Timing stability of TCSPC experiments

W. Becker, A. Bergmann

Proceedings Volume 6372, 637209 (2006) https://doi.org/10.1117/12.684750

KEYWORDS: Picosecond phenomena, Photons, Luminescence, Sensors, Microchannel plates, Error analysis, Single photon, Semiconductor lasers, Light sources, Electronics

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Proceedings Article | 25 October 2006 Paper

Paper

Differentiation of ocular fundus fluorophores by fluorescence lifetime imaging using multiple excitation and emission wavelengths

M. Hammer, D. Schweitzer, S. Schenke, W. Becker, A. Bergmann

Proceedings Volume 6372, 637202 (2006) https://doi.org/10.1117/12.684989

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Collagen, Picosecond phenomena, Diagnostics, Eye, Proteins, Auto-fluorescence imaging, Retina, Data modeling

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Proceedings Article | 25 October 2006 Paper

Paper

Time-resolved photon counting allows for new temporal and spatial insights into the nanoworld

Alexander Gaiduk, Ralf Kühnemuth, Suren Felekyan, Matthew Antonik, Wolfgang Becker, Volodymyr Kudryavtsev, Marcelle König, Fillip Oesterhelt, Carl Sandhagen, Claus Seidel

Proceedings Volume 6372, 637203 (2006) https://doi.org/10.1117/12.685366

KEYWORDS: Luminescence, Molecules, Fluorescence spectroscopy, Spectroscopy, Atomic force microscopy, Sensors, Fluorescence correlation spectroscopy, Molecular spectroscopy, Anisotropy, Signal detection

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Proceedings Article | 25 October 2006 Paper

Paper

Multiwavelength fluorescence lifetime imaging by TCSPC

Axel Bergmann, Wolfgang Becker

Proceedings Volume 6372, 637204 (2006) https://doi.org/10.1117/12.684743

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Sensors, Photodetectors, Fluorescence resonance energy transfer, Microscopes, Signal processing, Chromophores, Time correlated photon counting, Tissues

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Proceedings Article | 7 March 2006 Paper

Paper

In vivo autofluorescence lifetime imaging at the fundus of the human eye

Dietrich Schweitzer, Martin Hammer, Frank Schweitzer, Stefan Schenke, Eckhard Birckner, Wolfgang Becker, Axel Bergmann

Proceedings Volume 6138, 613808 (2006) https://doi.org/10.1117/12.644692

KEYWORDS: Luminescence, Picosecond phenomena, Eye, Fluorescence lifetime imaging, Mode conditioning cables, In vivo imaging, Laser scanners, Single photon, Oxygen, Fluorescence spectroscopy

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Proceedings Article | 23 February 2006 Paper

Paper

Spectrally resolved fluorescence lifetime and FRET measurements

Laimonas Kelbauskas, Sascha Dietrich, Birgit Hoffmann, Thomas Zimmer, Klaus Benndorf, Wolfgang Becker, Axel Bergmann, Nikolaj Klöcker, Christoph Biskup

Proceedings Volume 6089, 608903 (2006) https://doi.org/10.1117/12.645809

KEYWORDS: Luminescence, Fluorescence resonance energy transfer, Microscopes, Molecules, Sensors, Proteins, Streak cameras, Photons, Laser scanners, Time metrology

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Proceedings Article | 8 October 2005 Paper

Paper

Time- and wavelength-resolved autofluorescence detection by multi-dimensional TCSPC

Wolfgang Becker, Axel Bergmann, Christoph Biskup, Dietrich Schweitzer, Martin Hammer

Proceedings Volume 5862, 58620S (2005) https://doi.org/10.1117/12.633033

KEYWORDS: Luminescence, Eye, Fluorescence lifetime imaging, Tissues, Signal detection, Sensors, Picosecond phenomena, Ophthalmic imaging, Photodetectors, Skin

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Proceedings Article | 28 April 2005 Paper

Paper

Flying spot remote sensing of ICG kinetics of undeformed tissues

X. Wang, Z. Zhao, W. Becker, T. Troxler, B. Chance

Proceedings Volume 5693, (2005) https://doi.org/10.1117/12.610991

KEYWORDS: Breast, Signal detection, Absorption, Sensors, Photons, Luminescence, Remote sensing, Tissues, Imaging spectroscopy, Spectroscopy

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Proceedings Article | 28 April 2005 Paper

Paper

Multi-dimensional time-correlated single photon counting applied to diffuse optical tomography

Wolfgang Becker, Axel Bergmann, Adam Gibson, Nick Everdell, David Jennions, M. Schweiger, S. Arridge, Jeremy Hebden

Proceedings Volume 5693, (2005) https://doi.org/10.1117/12.589004

KEYWORDS: Sensors, Photons, Picosecond phenomena, Multiplexing, Luminescence, Absorption, Diffuse optical tomography, Tissue optics, Signal detection, Single photon

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Proceedings Article | 30 March 2005 Paper

Paper

Spectrally resolved fluorescence lifetime and FRET measurements

Christoph Biskup, Laimonas Kelbauskas, Thomas Zimmer, Sascha Dietrich, Klaus Benndorf, Wolfgang Becker, Axel Bergmann, Nikolaj Klocker

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.613006

KEYWORDS: Luminescence, Fluorescence resonance energy transfer, Molecules, Microscopes, Sensors, Photons, Proteins, Streak cameras, Time metrology, Signal processing

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Proceedings Article | 30 March 2005 Paper

Paper

Fluorescence lifetime images and correlation spectra obtained by multidimensional TCSPC

Wolfgang Becker, Axel Bergmann, Elke Haustein, Zdenek Petrasek, Petra Schwille, Christoph Biskup, Tiemo Anhut, Iris Riemann, Karsten Konig

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.588990

KEYWORDS: Luminescence, Fluorescence correlation spectroscopy, Fluorescence lifetime imaging, Sensors, Fluorescence resonance energy transfer, Microscopes, Proteins, Signal detection, Molecules, Picosecond phenomena

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SPIE Journal Paper | 1 July 2004 Open Access

Open Access

Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging

Christoph Biskup, Laimonas Kelbauskas, Thomas Zimmer, Klaus Benndorf, Axel Bergmann, Wolfgang Becker, J. Peter Ruppersberg, Clemens Stockklausner, Nikolaj Klöcker

JBO, Vol. 9, Issue 04, (July 2004) https://doi.org/10.1117/12.10.1117/1.1755721

KEYWORDS: Luminescence, Proteins, Resolution enhancement technologies, Molecules, Potassium, Green fluorescent protein, Visualization, Microscopes, Resonance energy transfer, Laser scanners

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Proceedings Article | 21 June 2004 Paper

Paper

High-speed FLIM data acquisition by time-correlated single-photon counting

Wolfgang Becker, Axel Bergmann, Giovanni Biscotti, Karsten Koenig, Iris Riemann, Laimonas Kelbauskas, Christoph Biskup

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.529113

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Sensors, Microscopes, Fluorescence resonance energy transfer, Picosecond phenomena, Laser scanners, Molecules, Proteins, Signal detection

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Proceedings Article | 1 June 2004 Paper

Paper

Advanced time-correlated single photon counting techniques for spectroscopy and imaging in biomedical systems

Wolfgang Becker, Axel Bergmann, Giovanni Biscotti, Angelika Rueck

Proceedings Volume 5340, (2004) https://doi.org/10.1117/12.529143

KEYWORDS: Luminescence, Photons, Sensors, Biomedical optics, Single photon, Signal detection, Imaging spectroscopy, Picosecond phenomena, Imaging systems, Fluorescence resonance energy transfer

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Proceedings Article | 10 July 2003 Paper

Paper

High resolution TCSPC lifetime imaging

Wolfgang Becker, Axel Bergmann, Christoph Biskup, Laimonas Kelbauskas, Thomas Zimmer, Nikolaj Klocker, Klaus Benndorf

Proceedings Volume 4963, (2003) https://doi.org/10.1117/12.472866

KEYWORDS: Sensors, Luminescence, Microscopes, Fluorescence lifetime imaging, Fluorescence resonance energy transfer, Photodetectors, Microchannel plates, Picosecond phenomena, Laser scanners, Modulation

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Proceedings Article | 17 June 2002 Paper

Paper

Lifetime imaging with the Zeiss LSM-510

Wolfgang Becker, Axel Bergmann, Georg Weiss

Proceedings Volume 4620, (2002) https://doi.org/10.1117/12.470706

KEYWORDS: Luminescence, Microscopes, Sensors, Picosecond phenomena, Fluorescence resonance energy transfer, Clocks, Laser scanners, Head, Nonlinear optics, Single photon

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Proceedings Article | 17 June 2002 Paper

Paper

Multiwavelength TCSPC lifetime imaging

Wolfgang Becker, Axel Bergmann, Christoph Biskup, Thomas Zimmer, Nikolaj Kloecker, Klaus Benndorf

Proceedings Volume 4620, (2002) https://doi.org/10.1117/12.470679

KEYWORDS: Luminescence, Microscopes, Sensors, Resolution enhancement technologies, Laser scanners, Clocks, Photodetectors, Time metrology, Signal processing, Proteins

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Proceedings Article | 2 November 2001 Paper

Paper

High-count-rate multichannel TCSPC for optical tomography

Wolfgang Becker, Axel Bergmann, Heidrun Wabnitz, Dirk Grosenick, Adam Liebert

Proceedings Volume 4431, (2001) https://doi.org/10.1117/12.447430

KEYWORDS: Sensors, Photons, Mammography, Tissue optics, Scattering, Optical spheres, Optical tomography, Absorption, Signal detection, Pulsed laser operation

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Proceedings Article | 2 November 2001 Paper

Paper

FRET measurements by TCSPC laser scanning microscopy

Wolfgang Becker, Klaus Benndorf, Axel Bergmann, Christoph Biskup, Karsten Koenig, Uday Tirlapur, Thomas Zimmer

Proceedings Volume 4431, (2001) https://doi.org/10.1117/12.447406

KEYWORDS: Fluorescence resonance energy transfer, Microscopes, Luminescence, Head, Laser scanners, Molecules, Microscopy, Single photon, Quenching (fluorescence), Sensors

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Proceedings Article | 24 April 2001 Paper

Paper

Picosecond fluorescence lifetime microscopy by TCSPC imaging

Wolfgang Becker, Axel Bergmann, Karsten Koenig, Uday Tirlapur

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424584

KEYWORDS: Microscopes, Luminescence, Sensors, Picosecond phenomena, Laser scanners, Single photon, Calcium, Imaging systems, Fluorescence resonance energy transfer, Microscopy

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Proceedings Article | 1 April 1996 Paper

Paper

High-speed electronics for the detection of time-resolved fluorescence in a continuous flow system

Rainer Erdmann, Uwe Ortmann, Joerg Enderlein, Wolfgang Becker, Michael Wahl, Edgar Klose

Proceedings Volume 2680, (1996) https://doi.org/10.1117/12.237635

KEYWORDS: Molecules, Luminescence, Photons, Picosecond phenomena, High speed electronics, Fluorescence spectroscopy, Time resolved spectroscopy, Molecular lasers, Laser induced fluorescence, Clocks

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Proceedings Article | 27 December 1995 Paper

Paper

Combined quantitative and qualitative two-channel optical biopsy technique for discrimination of tumor borders

Thomas Bocher, Juergen Beuthan, M. Scheller, Juergen Hopf, Marietta Linnarz, Rolf-Dieter Naber, Olaf Minet, Wolfgang Becker, Gerhard Mueller

Proceedings Volume 2627, (1995) https://doi.org/10.1117/12.228882

KEYWORDS: Tissues, Tumors, Luminescence, Tissue optics, Chromophores, In vivo imaging, Inflammation, Backscatter, Biopsy, Laser induced fluorescence

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Proceedings Article | 8 May 1995 Paper

Paper

Simultaneous detection of time-resolved emission spectra using a multianode PMT and new time-correlated single-photon counting (TCSPC) electronics with a 5-MHz count rate

Rainer Erdmann, Wolfgang Becker, Uwe Ortmann, Joerg Enderlein

Proceedings Volume 2388, (1995) https://doi.org/10.1117/12.208493

KEYWORDS: Picosecond phenomena, Sensors, Electronics, Luminescence, Single photon, Ultrafast phenomena, Monochromators, Time metrology, Oscilloscopes, Signal detection

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Proceedings Article | 22 December 1994 Paper

Paper

Very compact all solid state fluorescence lifetime measurement system: preliminary results

Rainer Erdmann, Gerald Kell, Rolf Krahl, Uwe Ortmann, Wolfgang Becker, Joerg Enderlein, Edgar Klose

Proceedings Volume 2328, (1994) https://doi.org/10.1117/12.197519

KEYWORDS: Picosecond phenomena, Luminescence, Sensors, Semiconductor lasers, Diodes, Electronics, Signal detection, Ultrafast phenomena, Single photon, Monochromators

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Proceedings Article | 21 July 1994 Paper

Paper

New compact TCSPC apparatus based on sub 15 ps laserdiodes: preliminary results

Rainer Erdmann, Gerald Kell, Wolfgang Becker, Edgar Klose

Proceedings Volume 2136, (1994) https://doi.org/10.1117/12.180798

KEYWORDS: Picosecond phenomena, Diodes, Luminescence, Ultrafast phenomena, Single photon, Laser development, Pulsed laser operation, Stray light, Sapphire lasers, Data acquisition

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Showing 5 of 63 publications

Proceedings Volume Editor (2)

SPIE Conference Volume | 11 October 2007

Advanced Photon Counting Techniques II

SPIE Conference Volume | 19 October 2006

Advanced Photon Counting Techniques

Conference Committee Involvement (21)

Multiphoton Microscopy in the Biomedical Sciences XXV

26 January 2025 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XXIV

28 January 2024 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XXIII

29 January 2023 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XXII

23 January 2022 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XXI

6 March 2021 | Online Only, California, United States

Multiphoton Microscopy in the Biomedical Sciences XX

2 February 2020 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIX

3 February 2019 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVIII

28 January 2018 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVII

29 January 2017 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVI

14 February 2016 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XV

8 February 2015 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIV

2 February 2014 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIII

3 February 2013 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XII

22 January 2012 | San Francisco, California, United States

Photon Counting Applications

19 April 2011 | Prague, Czech Republic

Multiphoton Microscopy in the Biomedical Sciences XI

23 January 2011 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences X

24 January 2010 | San Francisco, California, United States

Advanced Photon Counting Techniques III

14 April 2009 | Orlando, Florida, United States

Multiphoton Microscopy in the Biomedical Sciences IX

25 January 2009 | San Jose, California, United States

Advanced Photon Counting Techniques II

9 September 2007 | Boston, MA, United States

Advanced Photon Counting Techniques

1 October 2006 | Boston, Massachusetts, United States

Showing 5 of 21 Conference Committees

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