We resume a complex study of a new Coproporphyrin III preparation obtained in vitro via microbiological synthesis
in environment of a cultural medium of Arthrobacter globiformis with gaining of the coproporphyrin III tetrapotash salt
sterilized aqueous solution with 94.5% purity. The main impurities have porphyrinic nature.
The absorption spectrum shows that the preparation has multiple excitation bands in visible region with most pronounced
maxima at 501 nm, 535 nm, and 556 nm, and a weak one at 606 nm. The most effective spectral range of its
electronic states excitation can serve the region of a Soret band with maximum at 404 nm. Comparative luminescent and
photosensitizing properties of Coproporphyrin III and Fotoditazin preparations have been studied. Luminescence and
singlet oxygen quantum yields have been determined to be 0.03 and 0.37 (Coproporphyrin III) and 0.05 and 0.6 (Fotoditazin)
correspondingly. A reduction of the quantum yields values at an introduction of a biological additive into aqueous
solutions of the given preparations has been found.
Preclinical trials of the preparation are performed. It is shown that Coproporphyrin III toxicity value is LD50 = 2400 ± 120 mg/kg, that allows to rank this preparation to the (V) class of almost nontoxical medicinal agents. It is shown that
the preparation Coproporphyrin III has no teratogenic and allergenic properties and does not damage erythrocytes and
thrombocytes. Absence of an influence of the preparation on the blood flow speed has been established. The results obtained
thereby testify the absence of contra-indications for clinical tests of the Coproporphyrin III preparation on indicators
of sharp toxicity.
To study the effect of He-Ne irradiation (632.8 nm, 15 mW/cm2) on spontaneous contractive activity the fragments of rat portal vein weremounted isometrically in Krebs buffer. Irradiation of vessel fragments by He-Ne laser during 3,5 and 10 min caused the decrease of ton up to 50%, which lasted in postirradiation period (the observation time - 10 min). The frequency of phasic and tonic contractions did not change, but the amplitude increased up to 40% as compared to the initial level. The decreased basal tone level and the increased amplitude of phasic oscillations lasted in postirradiation period. Adding NO synthasa blocator (N - nitro-L-arginine) to Krebs solution before irradiation caused no significant changes mentioned above parameters. Irradiation and coputing of the same parameters of spontaneous contractive activity of vena porta caused no effects, mentioned in the absence of the blocator. From the results it is concluded that the decrease of tone is evoked by the increase of EDRF production and cGMP. The increase of amplitude of phasic and tonic contractions is connected with increase of Ca++ entry in every contraction cycle as a result of membrane Ca++ pool increase.
The effects of distant light diode irradiation with various spectrums of the trunk vessels on reactivity of microvessels in the small intestine mesentery treated with threshold doses of norepinephrine (NoE) are compared. The character of changes in reactivity of microvessels to NoE was found to depend on the wave length and irradiation dose. Ultraviolet irradiation (470 nm, 0.03 J/sm2) was noticed to increase reactivity of the vessels to NoE (vasoconstriction increase). In green light irradiation (540 nm, 0.3 J/sm2 sm2) no changes in reactivity were observed. Red light irradiation (670 nm, 2.0 J/sm2), infrared particular (980 nm, 1.0 J/sm2), lowered reactivity to NoE. Thus, noninvasive light-diode irradiation of the blood results in different systemic changes of endothelial dependent reactivity of microcirculation due to specify of photochemical processes involved.
Experimental laser thrombosis is induced in rat mesenteric microvessels. Temperature increase within irradiated microvessels was calculated on the basis of relatively simple heat transfer model and appeared to be near 50 degrees C above the initial value. This temperature increase decay practically to initial level during the period between two subsequent laser impulses. It is stated that the zone of endothelium thermal damage is less than thrombus length along the vessel wall.
Laser radiation has photomodification influence on blood and blood vessels. In our study we estimated the influence of distant laser irradiation of blood vessels.
Laser-induced thrombosis is one of the most adequate methods of studying of thrombus formation in mesenteric vessels. The in vivo simulation of different conditions of thrombi growth and the developed phenomenological theory of these processes confirm the concept of platelet activation time in treatment of the thrombi phenomenon.
Laser ((lambda) equals 0.630 micrometers , 1 - 3 mW/cm2 with exposition 10 min) was used to investigate the influence of laser irradiation on rat platelet functional activity. It was determined that irradiation of thrombocyte enriched plasma caused increase of ADP-induced thrombocytes aggregation. Disaggregation process was not influenced considerably by this irradiation.
The main factors in pathogenesis of the mouth mucosa and parodontium are defects in microcirculation: changes in the vessel tone, the vascular wall permeability and reactivity, rhealogical properties of blood and defects in metabolism and local factors of immunological defence. He-Ne laser (HNL) radiation with wavelength of 630 nm has been effectively used as an antiinflammatory procedure. HNL was determined to lower permeability of vessels, to increase the rate of volumetric blood flow, to rise Po2 in periodontal tissues which helps to stop tissue hypoxia and to do away with oedema.
Low energy laser radiation is widely used in different areas of medicine including dentistry. The main biologic laser radiation action mechanism is its multiangle influence on disease pathogenesis. In clinical practice, the most widely spread equipment is based on helium-neon laser (HNL) with the wavelength of 630 nm. HNL is able to cause different therapeutic effects depending on power flow density, i.e. antiinflammatory, microcirculation stimulation, metabolic, proliferative tissue activity, analgetic. However, considering specific features of oral cavity and parodontium disease pathogenesis, it is necessary to point to a few weak features of HNL. For example, HNL in therapeutic doses has insufficient antimicrobial and antiinflammatory effects; it just decreases microflora pathogeneity in the oral cavity. HNL radiation has poor lithic effect (for example, during the period of debridement) [Prokhonchukov, Zhizhina, Lapidus, 1985]. Regardless of the apparent positive HNL effect following extracorporal or intravascular blood irradiation in cases of generalized acute infection, this method is not used often in dentistry. Therefore, there is a necessity to search for some other more adequate laser irradiation sources which would solve prophylaxis and treatment problems for the most widely spread dental diseases such as caries, parodontosis. As far as a prior aim of laser radiation is to elicit different therapeutic effects, depending on laser beam intensity, the main principle of the treatment should be to vary this as well as wavelength values at different stages of development, duration and pathologic process profylaxis. In order to optimize efficiency of light biostimulators in dentistry it seems of importance to consider theoretical prerequisites of photobiological activation processes and specific activity of different light wavelength effects.
UV-laser irradiation has similar injuring effect when applied to arterioles and venules of the same diameter and may be used in the study of thrombogenic and thromboresistant properties of microvasculature. The thrombogenic potential of arterioles is greater than the same of venules but this difference under studied pathological processes tends to decrease (hypertension, hypoxia, tumor growth).
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