We present longitudinal functional activity imaging of liver functional zones in the presence of metabolic inhibitor drug with dynamic optical coherence tomography (DOCT). We studied a normal liver and a liver injected with metformin. In the normal liver, we found a correlation between the vascular structures observed in the DOCT and OCT projections. Over time, these vascular structures gradually decreased in the DOCT, while similar structures appeared in the OCT at a later time point. In the metformin-injected liver, we observed hexagonal liver lobule structures in the OCT. At late time point, several vessel-like structures with increased scattering appeared in the OCT, which were not initially present in the DOCT.
Tissue metabolism is an inter-and intra-cellular process, which is essential to maintain the living state of tissue. Functional abnormality of such cellular processes initiates several metabolic diseases including non-alcoholic-fatty-liver-disease (NAFLD), liver fibrosis, cancer, chronic kidney disease, and so on. To understand the metabolic processes and how tissue metabolism changes from healthy to diseased conditions, it is required to investigate tissue metabolic activity in animal models in a label-free manner. Since the metabolic structures in animal organs extend into the deep region, deep tissue metabolism imaging is necessary. In this paper, we demonstrate the investigation of ex vivo animal organ models’ metabolism including mouse liver and kidney tissues using OCT-based dynamics imaging method. The dynamics imaging method is based on logarithmic intensity variance (LIV) analysis that enables volumetric metabolic imaging with a 50-kHz swept-source OCT system. NAFLD model mouse livers and obstructed mouse kidney model were investigated ex vivo. In the normal liver, highly dynamic vessel-like structures were observed and they might correspond to high metabolic activity in the periportal/perivenous zones. In the 1-week NAFLD model, a macroscopic, ring-shaped dynamic structure was observed which may indicate highly-motile lipid droplets. In the 2-week NAFLD model, highly-dynamic fragmented vessel-like structures were observed, which are believed to correspond to inflammatory cells around large vessels. In the normal mouse kidney, superficial pipe-like metabolic structures were observed that may correspond to the kidney renal tubules. The pipe-like structures did not appear in the obstructed kidney model.
We present three-dimensional, label-free renal tubular metabolism imaging by functional optical coherence tomography (OCT) including dynamics imaging method so-called “logarithmic intensity variance (LIV)” and OCT angiography (OCTA). Normal mouse kidneys and obstructed kidney models were investigated ex vivo. In the normal kidney, several vertical tails of high-LIV and hyper-OCTA signals were observed in the corresponding cross-sectional images. These signals formed pipe-like structures in the en face slab average projection images. In the obstructed kidneys, such anatomical pipe-like structures disappeared and instead, a circular shell at the edge of the renal cortex region was observed in the LIV.
We present 3D intracellular motility imaging in MCD-diet induced non-alcoholic fatty liver disease (NAFLD) model by OCT-based dynamics imaging method, logarithmic intensity variance (LIV). LIV imaging visualizes the label-free intracellular activity. A 1-week and 2-week NAFLD model were investigated. In 1-week NAFLD, formation of large number of highly dynamic small particles at the beneath of the tissue surface were observed in LIV volume rendering image. In 2-week NAFLD model, a thin high LIV layer signal appeared in cross-sectional LIV image just beneath the tissue surface. The LIV projection and volume rendering images also reveal several discontinuous vessel-like structures.
We demonstrate label-free imaging of renal function with a unilateral ureteral obstruction (UUO) kidney mouse model. The imaging was performed by optical coherence microscopy which is capable of measuring tissue dynamics. Two different studies comprising of 1-week and 2-week UUO models were performed. A circular ring-shape high dynamics appearance at the periphery of the tissue surface was found in the 1-week UUO model for both obstructed and contralateral non-obstructed kidneys. In the 2-week UUO model, several vertical high dynamics regions were observed in cross-sectional dynamics images for both obstructed and non-obstructed kidneys. The results were validated by histological analysis.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.