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Dien K. Nguyen

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Proceedings Article | 24 February 2010 Paper

A 3-D fluorescence imaging system incorporating structured illumination technology

L. Antos, P. Emord, B. Luquette, B. McGee, D. Nguyen, A. Phipps, D. Phillips, M. Helguera

Proceedings Volume 7556, 75560L (2010) https://doi.org/10.1117/12.842609

KEYWORDS: Imaging systems, Calibration, Modulation, Luminescence, Spatial frequencies, Phase shifts, Image processing, 3D image processing, Light sources, Molecular imaging

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A currently available 2-D high-resolution, optical molecular imaging system was modified by the addition of a structured illumination source, Optigrid^TM, to investigate the feasibility of providing depth resolution along the optical axis. The modification involved the insertion of the Optigrid^TM and a lens in the path between the light source and the image plane, as well as control and signal processing software. Projection of the Optigrid^TM onto the imaging surface at an angle, was resolved applying the Scheimpflug principle. The illumination system implements modulation of the light source and provides a framework for capturing depth resolved mages. The system is capable of in-focus projection of the Optigrid^TM at different spatial frequencies, and supports the use of different lenses. A calibration process was developed for the system to achieve consistent phase shifts of the Optigrid^TM. Post-processing extracted depth information using depth modulation analysis using a phantom block with fluorescent sheets at different depths. An important aspect of this effort was that it was carried out by a multidisciplinary team of engineering and science students as part of a capstone senior design program. The disciplines represented are mechanical engineering, electrical engineering and imaging science. The project was sponsored by a financial grant from New York State with equipment support from two industrial concerns. The students were provided with a basic imaging concept and charged with developing, implementing, testing and validating a feasible proof-of-concept prototype system that was returned to the originator of the concept for further evaluation and characterization.

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