A 3-D fluorescence imaging system incorporating structured illumination technology

L. Antos; P. Emord; B. Luquette; B. McGee; D. Nguyen; A. Phipps; D. Phillips; M. Helguera

doi:10.1117/12.842609

23 February 2010 A 3-D fluorescence imaging system incorporating structured illumination technology

L. Antos, P. Emord, B. Luquette, B. McGee, D. Nguyen, A. Phipps, D. Phillips, M. Helguera

Proceedings Volume 7556, Design and Quality for Biomedical Technologies III; 75560L (2010) https://doi.org/10.1117/12.842609
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A currently available 2-D high-resolution, optical molecular imaging system was modified by the addition of a structured illumination source, Optigrid^TM, to investigate the feasibility of providing depth resolution along the optical axis. The modification involved the insertion of the Optigrid^TM and a lens in the path between the light source and the image plane, as well as control and signal processing software. Projection of the Optigrid^TM onto the imaging surface at an angle, was resolved applying the Scheimpflug principle. The illumination system implements modulation of the light source and provides a framework for capturing depth resolved mages. The system is capable of in-focus projection of the Optigrid^TM at different spatial frequencies, and supports the use of different lenses. A calibration process was developed for the system to achieve consistent phase shifts of the Optigrid^TM. Post-processing extracted depth information using depth modulation analysis using a phantom block with fluorescent sheets at different depths. An important aspect of this effort was that it was carried out by a multidisciplinary team of engineering and science students as part of a capstone senior design program. The disciplines represented are mechanical engineering, electrical engineering and imaging science. The project was sponsored by a financial grant from New York State with equipment support from two industrial concerns. The students were provided with a basic imaging concept and charged with developing, implementing, testing and validating a feasible proof-of-concept prototype system that was returned to the originator of the concept for further evaluation and characterization.

Citation Download Citation

L. Antos, P. Emord, B. Luquette, B. McGee, D. Nguyen, A. Phipps, D. Phillips, and M. Helguera "A 3-D fluorescence imaging system incorporating structured illumination technology", Proc. SPIE 7556, Design and Quality for Biomedical Technologies III, 75560L (23 February 2010); https://doi.org/10.1117/12.842609

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KEYWORDS

Imaging systems

Calibration

Modulation

Luminescence

Phase shifts

Spatial frequencies

Image processing

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