Minimal erythema dose (MED) is the amount of ultraviolet (UV) radiation needed to induce a mild skin erythema reaction after 24 hours following exposure. Determination of MED is based on the assessment of UV-erythema and traditionally performed visually by naked eye, which is subjective and connected with errors due to high intrarater and interrater variability. The application of non-invasive and quantitative techniques such as optical methods could improve MED calculation, allowing us to detect and quantify alterations in epidermis and dermis induced by UV irradiation. In the current study the analysis of microcirculation parameters by non-invasive optical methods revealed the relationship between the oxygen consumption and a dose of UV radiation. Results also showed the correlation of oxygen consumption of UV-exposed tissues normalized to intact skin with a dose of UV. Moreover, we described tendencies in dynamics of porphyrin fluorescence intensity at different time points after UV-exposure. Optical methods have some prospects in non-invasive and predictive evaluation of UV erythema and MED and more research should be conducted in this field.
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