Background:
The limitations of primary transurethral resection of bladder tumor (TURBt) have led the residual tumors rates as high as 75%. The intraoperative fluorescence imaging offers a great potential for improving TURBt have been
confirmed. So we aim to distinguish the residual tumors and normal mucosa using fluorescence molecular imaging
formed by conjugated molecule of the CSNRDARRC bladder cancer homing peptide with fluorescent dye. The
conjugated molecule was abbreviated FIuo-ACP. In our study, we will research the image features of FIuo-ACP
probe targeted bladder cancer for fluorescence molecular imaging diagnosis for bladder cancer in vivo and ex vivo.
Methods:
After the FIuo-ACP probe was synthetized, the binding sites, factors affecting binding rates, the
specificity and the targeting of Fluo-ACP labeled with bladder cancer cells were studied respectively
by laser scanning confocal microscope (LSCM), immunofluorescence and multispectral fluorescence
ex vivo optical molecular imaging system.
Results:
The binding sites were located in nucleus and the binding rates were correlated linearly with the dose
of probe and the grade of pathology. Moreover, the probe has a binding specificity with bladder
cancer in vivo and ex vivo. Tumor cells being labeled by the Fluo-ACP, bright green spots were
observed under LSCM. The tissue samples and tumor cells can be labeled and identified by
fluorescence microscope. Optical molecular imaging of xenograft tumor tissues was exhibited as
fluorescent spots under EMCCD.
Conclusion:
The CSNRDARRC peptides might be a useful bladder cancer targeting vector. The FIuo-ACP molecular probe was
suitable for fluorescence molecular imaging diagnosis for bladder cancer in vivo and ex vivo.
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