Mr. Yuan Tang Profile

Yuan Tang

at Florida International Univ

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Proceedings Article | 12 February 2010 Paper

The role of temperature increase rate in combinational hyperthermia chemotherapy treatment

Yuan Tang, Anthony McGoron

Proceedings Volume 7565, 75650C (2010) https://doi.org/10.1117/12.842587

KEYWORDS: Cell death, Luminescence, Cancer, Near infrared, Proteins, Heat treatments, Oncology, Indocyanine green, Tissue optics, Thermal modeling

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Hyperthermia in combination with chemotherapy has been widely used in cancer treatment. Our previous study has shown that rapid rate hyperthermia in combination with chemotherapy can synergistically kill cancer cells whereas a sub-additive effect was found when a slow rate hyperthermia was applied. In this study, we explored the basis of this difference. For this purpose, in vitro cell culture experiments with a uterine cancer cell line (MES-SA) and its multidrug resistant (MDR) variant MES-SA/Dx5 were conducted. P-glycoprotein (P-gp) expression, Caspase 3 activity, and heat shock protein 70 (HSP 70) expression following the two different modes of heating were measured. Doxorubicin (DOX) was used as the chemotherapy drug. Indocyanine green (ICG), which absorbs near infrared light at 808nm (ideal for tissue penetration), was chosen for achieving rapid rate hyperthermia. Slow rate hyperthermia was provided by a cell culture incubator. Two sets of thermal doses were delivered by either slow rate or rapid rate hyperthermia. HSP70 expression was highly elevated under low dose slow rate incubator hyperthermia while maintained at the baseline level under the other three treatments. Caspase3 level slightly increased after low dose slow rate incubator hyperthermia while necrotic cell death was found in the other three types of heat treatment. In conclusion, when given at the same thermal dose, slow rate hyperthermia is more likely to induce thermotolerance. Meanwhile, hyperthermia showed a dose dependent capability in reversing P-gp mediated MDR; when MDR is reversed, the combinational treatment induced extensive necrotic cell death. During this process, the rate of heating also played a very important role; necrosis was more dramatic in rapid rate hyperthermia than in slow rate hyperthermia even though they were given at the same dose.

Proceedings Article | 18 February 2009 Paper

Interaction of dye-enhanced photothermotherapy and chemotherapy in the treatment of cancer: an in vitro study

Yuan Tang, Anthony McGoron

Proceedings Volume 7164, 71640X (2009) https://doi.org/10.1117/12.808448

KEYWORDS: Cancer, Near infrared, Laser therapeutics, Ovarian cancer, In vitro testing, Tumors, Proteins, Oncology, Temperature metrology, Absorbance

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Doxorubicin (DOX), widely used in cancer chemotherapy, is limited by drug resistance and cardiac toxicity. Hyperthermia can aid the functionality of DOX, but current external heat delivery methods are hard to apply selectively and locally. Indocyanine green (ICG) absorbs near infrared light at 808nm (ideal for tissue penetration) and emits the energy as heat. These properties make it an ideal agent for rapid and localized hyperthermia. The purpose of this study was to investigate the in vitro cytotoxic effect of combined chemotherapy and hyperthermia to a DOX resistant ovarian cancer cell line (SKOV-3). The effects of laser-ICG photothermotherapy, which induces localized rapid heating, and an incubator, which induces a slow rate of heating, were compared. Cells were subjected to different concentrations of DOX and either 60 minutes in a 43°C incubator or to one minute at 43°C using 5μM of ICG and 808nm laser. SRB assay was used to measure cell growth. ICG itself without laser irradiation was not toxic to the cells. DOX by itself was cytotoxic with an IC₅₀ about 5μM. Both incubator and laser-ICG Hyperthermia in combination with DOX achieved significantly greater growth inhibition at all DOX concentrations compared to DOX alone. DOX combined with 60 minutes 43°C incubation lowered DOX IC₅₀ to about 1μM. The DOX IC₅₀ value with one minute laser-ICG was even lower (0.1μM) suggesting a synergistic effect between DOX and laser-ICG photothermotherapy. In conclusion, the combination of localized heating and chemotherapy may provide a valuable tool for cancer treatment with minimized toxic effect.

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