We reported a reusable DNA computing platform for solving satisfiability (SAT) problem based on surface
plamon resonance (SPR) technology in this paper. Three different sequences of 18-mer ssDNAs with thiol
terminal were first immobilized on the gold surface and then hybridized with their complementary sequences at
specific sites via microfluidic channels under room temperature. We also conjugated monoclonal antibody
(human IgG) to these complementary pairs chemically to amplify the hybridization signal and thus enhance the
noise margin to distinguish Boolean value of true and false. In order to keep the reaction temperature and SPR
measurement stable, repeated DNA annealing and denaturing is doned by varying salt concentration (by
adding NaOH to denature DNA) of reaction solution rather than changing reaction temperature. The
experimental results successfully demonstrated a multi-channel microfluidic DNA computation system to solve
a three variables (X, Y, Z) Boolean SAT problem (formula) with reusability and specificity
using protein-ssDNA conjugates to link to complementary ssDNA SAM surface under room temperature within
one hour. This technique provide a feasible solution to miniaturize the DNA computation platform for possible
iterated hyperstep computing processes.
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