A fluorescence-based device was developed for immunosuppressants. A measuring chip with ten parallel microchannels allows the simultaneous detection of more than one analyte with replicate measurements. The device is equipped with a microfluidic circuitry, which handles the sample mixing with necessary chemicals using an additional chip and its pumping into the measuring chip, and with integrated thin-film amorphous silicon photodiodes for the fluorescence detection. Submicrometric fluorescent magnetic particles are used to improve the efficiency of the assay. Results on the measurements of mycophenolic acid and cyclosporine A in both spiked solutions and microdialysate samples from patient blood are reported.
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