We constructed a high-speed laser line-scanning confocal microscope (LSCM) using He-Ne laser (633 nm), a line CCD
camera, and an acousto-optic deflector (AOD). The line scanner consists of an AOD and a cylindrical lens, which create
a line focus sweeping over the sample. The line scanner generates two-dimensional confocal images (512× 512 pixel
image) up to 191 frames per second with no mechanically-moving parts. This system is configured as an inverted
microscope for imaging biological organisms or tissues. Images of various biological samples were obtained including
rabbit cornea, onion cells, mouse melanoma tumor cells (B16BL6), and human breast tumor cells (BT-20). The frame
rate may be further improved up to over 700 frames per second when the image size is reduced (512×128 pixel image).
This system may be useful for analyzing fast phenomena during biological and chemical interactions and for imaging 3D
structures rapidly.
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