ECM stiffness is a potential instructive cue during capillary morphogenesis. Bulk measurements have often been used to investigate matrix stiffness as a cellular cue. However, local peri-cellular stiffness can be heterogeneous and vary greatly from measured bulk properties spatially and temporally. Here, we utilize bulk rheology and optical tweezers active microrheology (AMR) to investigate the dynamic mechanical crosstalk between cells and the surrounding matrix during capillary morphogenesis across length scales with various supporting stromal cells. We will present our current findings focusing on the discrepancies between bulk and microrheological distributions of measured stiffness across different stromal cell types.
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