A multimodal imaging system was developed, integrating swept-source optical coherence microscopy (SS-OCM) with a confocal fluorescence microscope for studying dry eye disease (DED). SS-OCM utilized a 1060 nm central wavelength source, resulting in 7.5 μm axial and 3.4 μm lateral resolution. The fluorescence subsystem employed a 488 nm laser with 2.4 μm lateral resolution. Simultaneous data acquisition at 100 kHz A-scan/pixel was achieved. The system allowed the characterization of the ocular surface's biochemical composition in vivo using fluorescently labeled ligands, facilitating visualization of adhering fluorescent molecules in animal models. Proof-of-concept experiments with a quantum dot-tagged ligand showed potential for improved DED diagnosis and therapeutic response evaluation.
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