Ex vivo distribution studies of macromolecular drugs, targets, and biomarkers have been typically limited to bulk sampling methods requiring tissue homogenization or 2D imaging. Bulk sampling cannot capture the complex spatial heterogeneity in tumors and 2D imaging techniques limit analysis to a few representative tissue sections. In this work we explored a tissue clearing, immunolabeling, and lightsheet imaging methods to visualize the ex vivo distribution of biologics in a preclinical models. Biologic distribution in intact tumors was assessed at sub-cellular resolution. A data handling and analysis workflow was developed to assess biologic penetration in tumors and influence of vascular heterogeneity. This work demonstrates potential applications to examine biologic drug distribution-efficacy relationships for drug discovery and development.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.