In a prospective observational pilot study, we evaluated label-free quantitative phase imaging (QPI) with digital holographic microscopy (DHM) as a tool to describe changes in biophysical properties of lymphocytes and monocytes after cardiac surgery. The results of our study show the capability of DHM to quantify perioperative lymphocyte and monocyte alternations in cardiac surgery patients. The patterns of biophysical DHM data correlated with laboratory parameters, flow cytometric cell markers, and postoperative course. This exemplifies DHM as a promising tool to characterize and assess inflammatory processes and course of disease.
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