We present a novel method of quantifying nitric oxide (NO) concentration, using ratiometric fluorescent nanosensors, during treatment with room-temperature plasma sources. Plasmas, which are ionized gases, generate copious amounts of reactive species and is widely used for different antimicrobial applications. These nanosensors comprise NO-responsive dye and perovskite nanocrystals encapsulated within hydrophobic biocompatible polymers, silica, and poly-lactic-co-glycolic acid (PLGA). They display dual emission at 510 nm, which is used as reference, and 586 nm, which is sensitive to NO concentration. These nanosensors can be useful for quantifying the NO production from different plasma sources and help with dose optimization.
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