Microorganisms can be found everywhere e.g. in food both as useful ingredients or harmful contaminations causing
food spoilage. Therefore, a fast and easy to handle analysis method is needed to detect bacteria in different kinds of
samples like meat, juice or air to decide if the sample is contaminated by harmful microorganisms. Conventional
identification methods in microbiology require always cultivation and therefore are time consuming.
In this contribution we present an analysis approach to identify fluorescence stained bacteria on strain level by means of
Raman spectroscopy. The stained bacteria are highlighted and can be localized easier against a complex sample
environment e.g. in food. The use of Raman spectroscopy in combination with chemometrical methods allows the
identification of single bacteria within minutes.
The identification of bacteria is necessary as fast as possible e.g. to provide an appropriate therapy for patients. Here the
cultivation time should be kept to a minimum. Beside microbiological identification methods Raman spectroscopy is a
valuable tool for bacteria identification. UV-resonance Raman spectroscopy enables selective monitoring of the cellular
DNA/RNA content and allows for a genotaxonomic classification of the bacteria. Since UV excitation may lead to
sample destruction the measurements are performed on rotated bacterial films.
For a faster identification avoiding the cultivation step single bacteria analysis is necessary. Using micro-Raman
spectroscopy a spatial resolution in the size range of the bacteria can be achieved. With this Raman excitation the
chemical components of the whole cell are measured which leads to a phenotypic classification. For localization of
bacteria inside complex matrices fluorescence labeling is achieved.
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