KEYWORDS: Tumors, Cancer detection, Blood, Biological samples, In vivo imaging, Near infrared, Biomedical optics, Light sources, Signal intensity, Photoacoustic microscopy
SignificanceWe developed a high-speed optical-resolution photoacoustic microscopy (OR-PAM) system using a high-repetition-rate supercontinuum (SC) light source and a two-axes Galvano scanner. The OR-PAM system enabled real-time imaging of optical absorbers inside biological tissues with excellent excitation wavelength tunability.AimIn the near-infrared (NIR) wavelength range, high-speed OR-PAM faces limitations due to the lack of wavelength-tunable light sources. Our study aimed to enable high-speed OR-PAM imaging of various optical absorbers, including NIR contrast agents, and validate the performance of high-speed OR-PAM in the detection of circulating tumor cells (CTCs).ApproachA high-repetition nanosecond pulsed SC light source was used for OR-PAM. The excitation wavelength was adjusted by bandpass filtering of broadband light pulses produced by an SC light source. Phantom and in vivo experiments were performed to detect tumor cells stained with an NIR contrast agent within flowing blood samples.ResultsThe newly developed high-speed OR-PAM successfully detected stained cells both in the phantom and in vivo. The phantom experiment confirmed the correlation between the tumor cell detection rate and tumor cell concentration in the blood sample.ConclusionsThe high-speed OR-PAM effectively detected stained tumor cells. Combining high-speed OR-PAM with molecular probes that stain tumor cells in vivo enables in vivo CTC detection.
KEYWORDS: Biological imaging, Signal detection, Contrast agents, Tumors, Tissues, Real time imaging, Veins, In vivo imaging, Photoacoustic microscopy, Dyes
To understand various phenomena that occur in living tissue, high-speed volume imaging technology that visualizes the dynamics of cells and molecules in living tissue is desired. For this purpose, we have developed a reflective MS-PAI with high excitation wavelength flexibility using a supercontinuum light source and bandpass filters, and a fast B-scan rate of over 100 fps. The ability of the MS-PAM for cell dynamics imaging was demonstrated by performing time-lapse volume imaging of contrast agent-stained cells flowing through blood vessels. We plan to combine the MS-PAM technology with functionalized contrast agents (imaging probes) to analyze dynamic phenomena.
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