The compatibility between coumarin-derived dendrimer (CdD)-captured silica particles (SiCdDs) and watersoluble
CdSe/ZnS quantum dots (QDs) in the FRET process improved the excited state of QDs in the reaction of singlet
oxygen production under LED irradiation. Sol-gel GA was successfully used to improve the binding between SiCdDs
and QDs. Singlet oxygen production using QDs coated with SiCdDs through sol-gel GA was enhanced by about 80 %
compared to that achieved using QDs only. The single oxygen produced by the QDs, the QDs/GA-SiCdDs complexes
and the SiCdDs/GA-QDs complexes in this study could be used in the treatment of HeLa cells.
CdSe/ZnS quantum dots (QDs) can be joined in the reductive pathway involving the electron transfer to an
acceptor or in the oxidative pathway involving the hole transfer to a donor. They were exploited in the oxidation
reactions of 5-aminolevulinic acid (ALA) and glutamate (GLU) for the generation of reactive oxygen species (ROS)
such as hydroxyl radical (HO●) and superoxide anion (O2● ─). Fast and highly efficient oxidation reactions of ALA to
produce HO● and of GLU to produce O2
●─ were observed in the cooperation of mercaptopropionic acid (MPA)-capped
CdSe/ZnS QDs under LED irradiation. Fluorescence spectroscopy and electron spin resonance (ESR) spectroscopy were
used to evaluate the generation of different forms of ROS. Confocal fluorescent microscopic images of the size and
morphology of HeLa cells confirmed the ROS generation from ALA or GLU in cooperation with CdSe/ZnS QDs under
LED irradiation.
In this study, a few optical immunosensors were developed to determine the concentration of BMP-7. Hydrophilic
CdSe/ZnS quantum dots (QDs) were synthesized and conjugated to the antibody of BMP-7 (BMP-7Ab). The QDconjugated
BMP-7Ab was used as a fluorescence probe at excitation and emission wavelengths of 470 nm and 585 nm,
respectively. It was immobilized either on the bottom of the well of a 96-well microtiter plate or on the tip of an optical
fiber. Two immunoassays, i.e. the direct and sandwich assays, were studied for their sensitivity. The sensitivity of the
direct immunoassay was 1296.21, compared to 384.69 for the sandwich assay. The linear detection range was 0.0-1.0
ng/mL for both assays. Based on the results of the microtiter plate technique, the direct assay technique was used for the
development of an optical fiber immunosensor. The optical fiber immunosensor has a linear detection range between 0.0
and 10.0 ng/mL with a detection limit of 0.413 ng/mL. The optical fiber immunosensor was applied to the sequential
injection analysis for the automatic determination of BMP-7.
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