For investigating the potential for personalized treatment of pancreatic cancer, two- and three-photon autofluorescence and second and third harmonic generation images were simultaneously generated from in vivo pancreatic tumor patient-derived xenograft (PDX) mice. Two different treatment regimens were administered to PDX mice for 5 weeks and compared. Control mice were treated only with saline. Mice were classified into three groups according to their response to the treatment: responsive, resistant, and control. Optical redox ratios were calculated from tumor regions which showed differences between the responsive group compared to the resistant and control groups.
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