Mr. Collin T. Inglut Profile

Collin T. Inglut

at Univ. of Maryland, College Park

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Publications (5)

Proceedings Article | 17 March 2023 Presentation

Excipient-free pure drug photosensitizer nanoparticles for PDT, imaging, and chemotherapy of cancer

John Quinlan, Collin Inglut, Idrisa Rahman, Huang Chiao Huang

Proceedings Volume PC12359, PC1235904 (2023) https://doi.org/10.1117/12.2655723

KEYWORDS: Photodynamic therapy, Nanoparticles, Cancer, Tumors, Optical imaging, Molecules, Brain cancer

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Proceedings Article | 24 May 2022 Presentation

Clinically relevant pure porphyrin nanoparticles for photodynamic therapy and priming applications

Collin Inglut, Jillian Stable, Brandon Gaitan, Wen-An Chiou, Baktiar Karim, Nina Connolly, Robert Robey, Graeme Woodworth, Michael Gottesman, Huang-Chiao Huang

Proceedings Volume PC12146, PC1214606 (2022) https://doi.org/10.1117/12.2618159

KEYWORDS: Photodynamic therapy, Nanoparticles, Tumors, Plasma display panels, Cancer, Brain, Photomedicine, Luminescence, Animal model studies, Tumor growth modeling

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Liposomes have revolutionized the field of photomedicine. Photodynamic therapy (PDT) using Visudyne®, a liposomal photosensitizer formulation, has helped many patients globally. Since the FDA approved Visudyne® in 2002, countless studies have examined strategies to further improve the therapeutic index of lipid-based photosensitizing nanoconstructs. While liposomes can improve the pharmacokinetics of hydrophobic photosensitizers, they could also modulate cellular uptake and singlet oxygen production. Furthermore, it is evident that there are other immunological and toxicological considerations for the design of liposomal drugs. Accordingly, there is now an emerging trend to engineer carrier-free nanodrugs. Here, we developed a pure-drug nanoparticle using the clinically used verteporfin photosensitizer (termed nanoVP) for photodynamic applications. We validated the effects of nanoVP in three contexts: 1) cytotoxic PDT, 2) subtherapeutic PDT, and 3) dark toxicity. Using a brain cancer murine model, we showed that light activation of nanoVP reduced tumor volume by up to 54% compared to liposomal VP. Fluorescence imaging revealed that nanoVP had a superior tumor-to-liver tissue ratio (~0.92) compared to liposomal VP (~0.4). We further studied nanoVP-mediated PDT at subtherapeutic doses to achieve photodynamic priming (PDP). PDP has been shown to enhance drug delivery, activate antitumor immunity, and sensitize tumors to chemotherapy. This approach is particularly relevant in the brain, where high doses of PDT can result in edema, neurotoxicity, and even animal death. Using a rat model, we demonstrated that nanoVP-assisted PDP improved blood-brain barrier permeability and accumulation of a model drug (Evans Blue dye) in rat brains by >5 fold. Minimal to no brain damage was observed. Lastly, under dark conditions, we validated that nanoVP significantly reduced viability while liposomal VP stimulated cancer cell growth. Results from this work demonstrate the utility of nanoVP for cancer treatment. The development of pure-drug photosensitizing nanoparticles for photodynamic applications could further revolutionize the field of photomedicine.

SPIE Journal Paper | 26 September 2020 Open Access

Depth-resolved imaging of photosensitizer in the rodent brain using fluorescence laminar optical tomography

Brandon Gaitan, Collin Inglut, Yi Liu, Yu Chen, Huang-Chiao Huang

JBO, Vol. 25, Issue 09, 096007, (September 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.9.096007

KEYWORDS: Brain, Tissues, Neuroimaging, Luminescence, Signal attenuation, Imaging systems, Optical tomography, Capillaries, 3D image processing, Optical coherence tomography

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Proceedings Article | 20 April 2020 Presentation

Using fluorescence laminar optical tomography to measure the distribution photodynamic drug in the brain to optimize dosage and treatment time (Conference Presentation)

Brandon Gaitan, Collin Inglut, Yu Chen, Huang-Chiao Huang

Proceedings Volume 11219, 112190J (2020) https://doi.org/10.1117/12.2550498

KEYWORDS: Luminescence, Brain, Optical tomography, Optical testing, Plasma display panels, Tissue optics, Tumors, Brain cancer, Blood brain barrier, Oxygen

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Proceedings Article | 14 August 2019 Presentation

Photosensitizing biomolecules and nanocrystals for anti-glioma photodynamic therapy (Conference Presentation)

Collin Inglut, Yan Baglo, Barry Liang, Jill Stabile, Yahya Cheema, Graeme Woodworth, Huang-Chiao Huang

Proceedings Volume 11070, 110701M (2019) https://doi.org/10.1117/12.2525904

KEYWORDS: Nanocrystals, Photodynamic therapy, Biomedical optics, Polymers, Macromolecules, Crystals, Photosensitizer targeting

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