We present a ready to use label free coherent Raman microscope (CARS/SRS) to perform instantaneous virtual histology and chemical imaging for scientific applications. The system is based on a picosecond laser source that can address two vibrational wavenumbers simultaneously across the entire vibrational spectrum (400cm-1 – 4000cm-1). The laser source is coupled to a ready to use beam scanning microscope enabling SRS, CARS and multiphoton microscopy. Targeting the CH2 and CH3 chemical bonds, we demonstrate instantaneous stimulated Raman histology in the brain and the GI. This new, ready to use, SRS/CARS and multiphoton microscope enables SRS detection with exquisite sensitivity (shot noise limited) and ratio-metric measurement by targeting simultaneously two vibrational frequencies that can be tuned anywhere across the vibrational spectrum.
Diagnostic genomic profiling constitutes one of the major challenges to cure brain tumors. The deployment of such analyses depends on the quality of the surgical specimen sent for histopathological examination and further molecular studies. The aim of our study was to assess the potential added value of Stimulated Raman Histology (SRH) for the assessment of freshly excised central nervous system samples. We showed that SRH enabled a near-instant microscopic examination of various central nervous system samples without any tissue processing such as labelling, freezing nor sectioning. Following SRH imaging, we demonstrated that the samples could be readily recovered and reintroduced into a conventional pathology workflow including immunohistochemistry and genomic profiling to establish a definitive diagnosis.
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