Mr. Bin Wang Profile

Bin Wang

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 23 February 2009 Paper

Multifunctional sensing film used for fiber optic cholesterol sensor

Bin Wang, Jun Huang

Proceedings Volume 7278, 72780Q (2009) https://doi.org/10.1117/12.821321

KEYWORDS: Fiber optics sensors, Sensors, Fiber optics, Luminescence, Oxygen, Sol-gels, Catalysis, Biosensors, Biosensing, Bioalcohols

Read Abstract +

In this paper, by using ethyl silicate, ethanol and fluorescence indicator as the precursors, the multifunctional optic biosensing (MOBS) film containing cholesterol oxidase and the fluorescence indicator was prepared by sol-gel method. This biosensing film has both the function of biocatalyst and oxygen biosensing and can be used as the effective biosensing materials for fiber optic cholesterol sensor. The fiber optical cholesterol sensor based on fluorescence quenching was designed and fabricated using lock-in amplifying technology to realize the detection of cholesterol concentration. The experimental results showed that the best precursor proportion in volume ratio is: ethyl silicate: ethanol: 0.01 M HCl = 5: 8: 1.6. The drying rate of the sol could be controlled by using formamide as the controlling drier. When 16% of formamide were added in the mixing system, the cracks of the film could be reduced greatly and the immobilization of cholesterol oxidase and the fluorescence indicator could be improved effectively. A linear relationship between phase delay φ and the cholesterol concentration was observed in the range of 100 to 500 mg/dL. Since the cholesterol concentration is in the range of 140 to 200 mg/dL in the blood of healthy people, it will be possible for the sensor to be used in clinical detection. The biosensor with MOBS film has the response time of about 30 s, which is rather fast for a biosensor, and the relative deviation of ±5.03%. This biosensor also has good stability.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years