Photoacoustic microscopy of arteriovenous shunts and blood diffusion in early-stage tumors

Chenghung Yeh; Jinyang Liang; Yong Zhou; Song Hu; Rebecca E. Sohn; Jeffrey M. Arbeit; Lihong V. Wang

doi:10.1117/1.JBO.21.2.020501

16 February 2016 Photoacoustic microscopy of arteriovenous shunts and blood diffusion in early-stage tumors

Chenghung Yeh, Jinyang Liang, Yong Zhou, Song Hu, Rebecca E. Sohn, Jeffrey M. Arbeit, Lihong V. Wang

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Journal of Biomedical Optics, Vol. 21, Issue 2, 020501 (February 2016). https://doi.org/10.1117/1.JBO.21.2.020501

Abstract

Angiogenesis in a tumor region creates arteriovenous (AV) shunts that cause an abnormal venous blood oxygen saturation (sO₂) distribution. Here, we applied optical-resolution photoacoustic microscopy to study the AV shunting in vivo. First, we built a phantom to image sO₂ distribution in a vessel containing converged flows from two upstream blood vessels with different sO₂ values. The phantom experiment showed that the blood from the two upstream vessels maintained a clear sO₂ boundary for hundreds of seconds, which is consistent with our theoretical analysis using a diffusion model. Next, we xenotransplanted O-786 tumor cells in mouse ears and observed abnormal sO₂ distribution in the downstream vein from the AV shunts in vivo. Finally, we identified the tumor location by tracing the sO₂ distribution. Our study suggests that abnormal sO₂ distribution induced by the AV shunts in the vessel network may be used as a new functional benchmark for early tumor detection.

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Chenghung Yeh, Jinyang Liang, Yong Zhou, Song Hu, Rebecca E. Sohn, Jeffrey M. Arbeit, and Lihong V. Wang "Photoacoustic microscopy of arteriovenous shunts and blood diffusion in early-stage tumors," Journal of Biomedical Optics 21(2), 020501 (16 February 2016). https://doi.org/10.1117/1.JBO.21.2.020501

Published: 16 February 2016

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