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Here, we present a rapid dual-view projection imaging method with two-photon glutamate uncaging capability based on an upright Bessel beam light-sheet microscopy setup. Compared with light-sheet imaging, our projection imaging method can significantly improve the volumetric rate for neural imaging. In addition, an independent laser scanning module is designed for 2P uncaging, allowing simultaneous synaptic resolution of stimulation and 100 Hz volumetric imaging of neural activity in deep tissue. Imaging results from mouse brain slices under 2P glutamate uncaging will be presented.
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Dongli Xu, Jun B. Ding, Leilei Peng, "Rapid dual-views projection microscopy with two-photon glutamate uncaging for neural imaging," Proc. SPIE PC12828, Neural Imaging and Sensing 2024, PC1282803 (13 March 2024); https://doi.org/10.1117/12.3003734