Prof. Leilei L. Peng Profile

Prof. Leilei L. Peng

at Wyant College of Optical Sciences

SPIE Involvement:

Conference Program Committee | Author

Proceedings Article | 13 March 2024 Presentation

Rapid dual-views projection microscopy with two-photon glutamate uncaging for neural imaging

Dongli Xu, Jun Ding, Leilei Peng

Proceedings Volume PC12828, PC1282803 (2024) https://doi.org/10.1117/12.3003734

KEYWORDS: Microscopy, 3D projection, Stereoscopy, Brain tissue, Microscopes, Imaging systems, Bessel beams, Two photon imaging, Temporal resolution, Neurons

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Proceedings Article | 28 April 2022 Presentation

Rapid random z-access two-photon light-sheet microscope for neural imaging

Dongli Xu, Jun Ding, Leilei Peng

Proceedings Volume PC11946, PC119460O (2022) https://doi.org/10.1117/12.2610667

KEYWORDS: Microscopes, Tissue optics, Stereoscopy, Visualization, Video, Neuroimaging, Microscopy, Luminescence, Fluorescence spectroscopy, Calibration

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Proceedings Article | 5 March 2021 Presentation

Deep tissue light sheet imaging with structured illumination

Leilei Peng

Proceedings Volume 11657, 116570B (2021) https://doi.org/10.1117/12.2579295

KEYWORDS: Tissue optics, Tissues, Scattering, Light scattering, Deep tissue imaging, Super resolution, Stereoscopy, Optical resolution, Optical imaging, Neuroimaging

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Proceedings Article | 9 March 2020 Presentation

Two-photon Bessel beam scanning microscope for neural activities (Conference Presentation)

Dongli Xu, Leilei Peng, Jun Ding

Proceedings Volume 11226, 1122603 (2020) https://doi.org/10.1117/12.2546526

KEYWORDS: Microscopes, Bessel beams, 3D image reconstruction, Tissue optics, Brain, Tissues, Stereoscopy, Neuroscience, Temporal resolution, In vivo imaging

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Proceedings Article | 24 April 2017 Presentation

Parallel-multiplexed excitation light-sheet microscopy (Conference Presentation)

Dongli Xu, Weibin Zhou, Leilei Peng

Proceedings Volume 10076, 100760A (2017) https://doi.org/10.1117/12.2253250

KEYWORDS: Fluorescent markers, Bessel beams, Hyperspectral systems, Fluorescent proteins, Multiplexing, Microscopes, Microscopy, Laser scanners, Stereoscopy, 3D image processing

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Proceedings Article | 19 April 2017 Presentation

High resolution multiplexed functional imaging in live embryos (Conference Presentation)

Dongli Xu, Weibin Zhou, Leilei Peng

Proceedings Volume 10043, 100430M (2017) https://doi.org/10.1117/12.2252818

KEYWORDS: Functional imaging, Fluorescence resonance energy transfer, Imaging systems, Bessel beams, Multiplexing, Fluorescence lifetime imaging, 3D image processing, Image resolution, Laser optics, Optical tomography

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Proceedings Article | 27 April 2016 Presentation

Bessel beam fluorescence lifetime tomography of live embryos (Conference Presentation)

Dongli Xu, Leilei Peng

Proceedings Volume 9716, 97160H (2016) https://doi.org/10.1117/12.2213972

KEYWORDS: Bessel beams, Imaging systems, Optical tomography, Luminescence, Tomography, Image processing, Fluorescence tomography, Stereoscopy, Fluorescence lifetime imaging, Image resolution

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Proceedings Article | 26 April 2016 Presentation

High resolution multiplexed functional imaging in live embyros (Conference Presentation)

Dongli Xu, Leilei Peng

Proceedings Volume 9700, 97000U (2016) https://doi.org/10.1117/12.2213994

KEYWORDS: Image resolution, Optical tomography, Bessel beams, Multiplexing, Functional imaging, Tomography, Tissue optics, Gaussian beams, Algorithm development, Imaging systems

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Proceedings Article | 22 February 2013 Paper

Nonlinear structured illumination microscopy with surface plasmon resonance enhanced stimulated emission depletion

Han Zhang, Ming Zhao, Leilei Peng

Proceedings Volume 8590, 859011 (2013) https://doi.org/10.1117/12.2005043

KEYWORDS: Stimulated emission depletion microscopy, Microscopy, Signal to noise ratio, Image resolution, Resonance enhancement, Super resolution, Surface plasmons, Luminescence, Stochastic processes, Microscopes

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Proceedings Article | 28 February 2006 Paper

Phase-contrast BioCD: high-speed immunoassays at sub-picogram detection levels

Ming Zhao, Leilei Peng, W. Cho, F. Regnier, D. Nolte

Proceedings Volume 6095, 60950L (2006) https://doi.org/10.1117/12.646827

KEYWORDS: Proteins, Signal detection, Interferometry, Sensors, Optical lithography, Coating, Printing, Adaptive optics, Phase contrast, Phase shift keying

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Proceedings Article | 1 April 2005 Paper

The adaptive BioCD: interferometric immunoassay on a spinning disk

Leilei Peng, Manoj Varma, Fred Regnier, David Nolte

Proceedings Volume 5692, (2005) https://doi.org/10.1117/12.589217

KEYWORDS: Homodyne detection, Proteins, Interferometry, Phase modulation, Phase shift keying, Glasses, Laser beam diagnostics, Oscillators, Calibration, Adaptive optics

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Proceedings Article | 29 March 2005 Paper

Label-free multi-analyte detection using a BioCD

Manoj Varma, Leilei Peng, Fred Regnier, David Nolte

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.591633

KEYWORDS: Gold, Proteins, Interferometry, Sensors, Image segmentation, Signal detection, Biological research, Interferometers, Mirrors, Molecules

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We previously reported the application of spinning-disk interferometry, implemented in a compact optical sensor format called the BioCD, in the detection of antigen-antibody recognition. The BioCD consists of interferometers micro-fabricated on the surface of a 2” laser mirror disk, which can spin up to 6000 rpm resulting in high data acquisition rates. The interferometric elements are fabricated by evaporating gold ridges on the mirror substrate operating in the linear sensitivity regime of the interferometer defined as quadrature. Antibodies or proteins are immobilized on the gold interferometric structures through alkanethiols, and the target molecules are immobilized by application of reagents or samples to the disk while it is spinning. The centrifugal force distributes the sample over the sensor surface, causing a change in the optical phase of the interferometric elements, which is detected in real time using a lock-in amplifier with small detection bandwidth. We detected the binding of Mouse IgG by immobilized Anti-Mouse IgG using the BioCD with a detection limit of 1 ng/ml and low non-specific binding. Furthermore, the selectivity of specific binding was found to be greater than 1 in 10000, determined using the response curve of the BioCD to exposures of specific and non-specific analytes of varying concentrations. This opens up the possibility of simultaneous detection of several analytes with the same sensor while maintaining high selectivity. In this paper we demonstrate simultaneous detection of Rabbit and Mouse IgG on the same disk. The sensitivity limit for multi-analyte detection remains the same as that for a single analyte. In addition to the ability to do simultaneous detection, the current detection scheme presents a way to reference the results of one track with respect to others, thus increasing the reliability of the data. Used in conjunction with high-density protein patterning techniques, the BioCD has the potential to be a highly multiplexed label-free high-speed sensor.

Proceedings Article | 22 June 2004 Paper

Spinning-disk laser interferometers for immuno-assays and proteomics: the BioCD

David Nolte, Manoj Varma, Leilei Peng, Halina Inerowicz, Fred Regnier

Proceedings Volume 5328, (2004) https://doi.org/10.1117/12.529482

KEYWORDS: Interferometers, Gold, Adaptive optics, Interferometry, Reflectivity, Molecules, Dielectrics, Near field, Phase modulation, Near field optics

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Proceedings Article | 8 July 2003 Paper

Imaging of tumor necroses using full-frame optical coherence imaging

Ping Yu, L. Peng, M. Mustata, David Nolte, J. Turek, Michael Melloch, Christopher Dunsby, Yan Gu, Paul French

Proceedings Volume 4956, (2003) https://doi.org/10.1117/12.477823

KEYWORDS: Tumors, Holograms, Holography, Coherence imaging, Speckle, Tissue optics, Optical coherence tomography, CCD cameras, Four wave mixing, Biomedical optics

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Showing 5 of 14 publications

Conference Committee Involvement (5)

Neural Imaging and Sensing 2025

27 January 2025 | San Francisco, California, United States

Neural Imaging and Sensing 2024

27 January 2024 | San Francisco, California, United States

Neural Imaging and Sensing 2023

30 January 2023 | San Francisco, California, United States

Neural Imaging and Sensing 2022

24 January 2022 | San Francisco, California, United States

Neural Imaging and Sensing 2021

6 March 2021 | Online Only, California, United States

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