Interface self-referencing dynamic full-field optical coherence tomography for 3D live imaging of samples on glass slides

Tual Monfort; Salvatore Azzollini; Kate Grieve; Olivier Thouvenin

doi:10.1117/12.2652207

9 March 2023 Interface self-referencing dynamic full-field optical coherence tomography for 3D live imaging of samples on glass slides

Tual Monfort, Salvatore Azzollini, Kate Grieve, Olivier Thouvenin

Author Affiliations +

Proceedings Volume PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII; PC123671E (2023) https://doi.org/10.1117/12.2652207
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present interface self-referencing D-FF-OCT (iSR-D-FF-OCT), a new dynamic full-field optical coherence tomography (D-FF-OCT) imaging technique, which is self-referenced, vibration-insensitive and overcomes D-FF-OCT fringe artefact limitations near specular surfaces. iSR-(D)-FF-OCT complements and is easily implemented on existing D-FF-OCT set-ups to cover a zero to several hundred micrometer depth range. iSR-(D)-FF-OCT has a homogeneous contrast across the imaging field enabling flawless mosaicking. In fibroblasts, retinal organoids, and corneal explants, we demonstrate imaging in the vicinity of reflective interfaces without fringe artefacts, in a live, label-free, non-invasive manner with sub-diffraction limited sectioning capacity within 3D samples.

Conference Presentation

Citation Download Citation

Tual Monfort, Salvatore Azzollini, Kate Grieve, and Olivier Thouvenin "Interface self-referencing dynamic full-field optical coherence tomography for 3D live imaging of samples on glass slides", Proc. SPIE PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII, PC123671E (9 March 2023); https://doi.org/10.1117/12.2652207

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