Fluorescence detected Mid infrared photothermal microscopy

Yi Zhang; Ji-Xin Cheng; Haonan Zong; Cheng Zong; Meng Zhang; Yuying Tan; Yuewei Zhan; Danchen Jia

doi:10.1117/12.2606441

2 March 2022 Fluorescence detected Mid infrared photothermal microscopy

Yi Zhang, Ji-Xin Cheng, Haonan Zong, Cheng Zong, Meng Zhang, Yuying Tan, Yuewei Zhan, Danchen Jia

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730M (2022) https://doi.org/10.1117/12.2606441
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present a novel version of mid-infrared photothermal microscopy in which thermosensitive fluorescent probes are harnessed to sense the mid-infrared photothermal effect. The fluorescence intensity can be modulated at the level of 1% per Kelvin, which is 100 times larger than the modulation of scattering intensity. In addition, fluorescence emission is free of interference, thus much improving the image quality. Moreover, fluorophores can target specific organelles or biomolecules, thus augmenting the specificity of photothermal imaging. Spectral fidelity is confirmed by fingerprinting a single bacterium. A wide-field fluorescence-detected mid-infrared photothermal microscope developed allows video-rate bond-selective imaging of biological specimens.

Conference Presentation

Citation Download Citation

Yi Zhang, Ji-Xin Cheng, Haonan Zong, Cheng Zong, Meng Zhang, Yuying Tan, Yuewei Zhan, and Danchen Jia "Fluorescence detected Mid infrared photothermal microscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730M (2 March 2022); https://doi.org/10.1117/12.2606441

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