Study on distinction of particles and bubbles for particle counting in liquids by the use fluorescence

Takayuki Sakaguchi; Kensei Ehara

doi:10.1117/12.501013

30 May 2003 Study on distinction of particles and bubbles for particle counting in liquids by the use fluorescence

Takayuki Sakaguchi, Kensei Ehara

Proceedings Volume 5144, Optical Measurement Systems for Industrial Inspection III; (2003) https://doi.org/10.1117/12.501013
Event: Optical Metrology, 2003, Munich, Germany

Abstract

In order to find conditions that no bubbles occur on particle counting in liquid, the use of simultaneous detection of scattered light and fluorescence was examined. A liquid particle counter that had fluorescence sensing devices to detect the fluorescence from sample particles was used. Sample particles were dyed and from 1 to 10 micrometer in diameter. The wavelength of laser light to excite fluorescence was 488nm. The fluorescence was filtered by a dichroic mirror and two band pass color filters and detected at 525nm. Although ordinary particle counters detect only scattered light, our system is capable of detecting coincidence of scattered light and fluorescence. By making use of the fact that bubbles make no coincidence signals and particles make coincidence signals we can distinguish bubbles from particles. To evaluate the reliability of the particle counts obtained with this system, we compared them with the counts obtained with the microscopic method.

Citation Download Citation

Takayuki Sakaguchi and Kensei Ehara "Study on distinction of particles and bubbles for particle counting in liquids by the use fluorescence", Proc. SPIE 5144, Optical Measurement Systems for Industrial Inspection III, (30 May 2003); https://doi.org/10.1117/12.501013

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