Dual-wavelength excitation multispectral autofluorescence lifetime imaging handheld probe for label-free metabolic clinical imaging of the oral mucosa

Gabriel P. Tortorelli; Rodrigo Cuenca Martinez; Michael J. Serafino; Wihan Kim; Brian Applegate; Javier A. Jo

doi:10.1117/12.2608936

7 March 2022 Dual-wavelength excitation multispectral autofluorescence lifetime imaging handheld probe for label-free metabolic clinical imaging of the oral mucosa

Gabriel P. Tortorelli, Rodrigo Cuenca Martinez, Michael J. Serafino, Wihan Kim, Brian Applegate, Javier A. Jo

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Proceedings Volume PC11935, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2022; PC1193508 (2022) https://doi.org/10.1117/12.2608936
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Increased cellular metabolic activity, a hallmark of malignant epithelial cells, can be quantified by imaging the oral tissue autofluorescence originated from the metabolic cofactors NADH and FAD. We report a novel multispectral autofluorescence lifetime imaging (maFLIM) handheld probe capable of simultaneous autofluorescence excitation at 375 nm (for NADH) and 445 nm (for FAD), and simultaneous multispectral time-resolved fluorescence measurement at four emission spectral channels. The performance of the dual-wavelength excitation maFLIM handheld probe was assessed by imaging fluorescent dye standards with well characterized fluorescence lifetimes, and the oral mucosa of human subjects in oral health care settings.

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Gabriel P. Tortorelli, Rodrigo Cuenca Martinez, Michael J. Serafino, Wihan Kim, Brian Applegate, and Javier A. Jo "Dual-wavelength excitation multispectral autofluorescence lifetime imaging handheld probe for label-free metabolic clinical imaging of the oral mucosa", Proc. SPIE PC11935, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2022, PC1193508 (7 March 2022); https://doi.org/10.1117/12.2608936

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Multispectral imaging

Mirrors

Lenses

Human subjects

Luminescence

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