Low level light in combination with metabolic modulators for effective therapy

Tingting Dong; Qi Zhang; Michael R. Hamblin; Mei X. Wu M.D.

doi:10.1117/12.2079363

5 March 2015 Low level light in combination with metabolic modulators for effective therapy

Tingting Dong, Qi Zhang, Michael R. Hamblin, Mei X. Wu M.D.

Proceedings Volume 9309, Mechanisms for Low-Light Therapy X; 93090A (2015) https://doi.org/10.1117/12.2079363
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Vascular damage occurs frequently at the injured brain causing hypoxia and is associated with poor outcomes in the clinics. We found high levels of glycolysis, reduced ATP generation, and increased formation of reactive oxygen species (ROS) and apoptosis in neurons under hypoxia. Strikingly, these adverse events were reversed significantly by noninvasive exposure of injured brain to low-level light (LLL). LLL illumination sustained the mitochondrial membrane potential, constrained cytochrome C leakage in hypoxic cells, and protected them from apoptosis, underscoring a unique property of LLL. The effect of LLL was further bolstered by combination with metabolic substrates such as pyruvate or lactate both in vivo and in vitro. The combinational treatment retained memory and learning activities of injured mice to a normal level, whereas those treated with LLL or pyruvate alone, or sham light displayed partial or severe deficiency in these cognitive functions. In accordance with well-protected learning and memory function, the hippocampal region primarily responsible for learning and memory was completely protected by a combination of LLL and pyruvate, in marked contrast to the severe loss of hippocampal tissue due to secondary damage in control mice. These data clearly suggest that energy metabolic modulators can additively or synergistically enhance the therapeutic effect of LLL in energy-producing insufficient tissues like injured brain. Keywords:

Citation Download Citation

Tingting Dong, Qi Zhang, Michael R. Hamblin, and Mei X. Wu M.D. "Low level light in combination with metabolic modulators for effective therapy", Proc. SPIE 9309, Mechanisms for Low-Light Therapy X, 93090A (5 March 2015); https://doi.org/10.1117/12.2079363

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available