New cross-linking quinoline- and quinolone-based luminescent lanthanide probes for sensitive labeling

Shyamala Pillai; Laura Wirpsza; Maxim Kozlov; Salvatore A. E Marras; Lev N. Krasnoperov; Arkady Mustaev

doi:10.1117/12.921056

2 February 2012 New cross-linking quinoline- and quinolone-based luminescent lanthanide probes for sensitive labeling

Shyamala Pillai, Laura Wirpsza, Maxim Kozlov, Salvatore A. E Marras, Lev N. Krasnoperov, Arkady Mustaev

Proceedings Volume 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV; 82331C (2012) https://doi.org/10.1117/12.921056
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

New luminescent lanthanide chelates containing thiol-, amine-, and click-reactive groups in antenna-fluorophore moieties were synthesized. The chelates include diethylenetriaminepentaacetic acid (DTPA) coupled to two types of chromophores: 7-amino-4-trifluoromethyl-2(1H) quinolinone, and 7-amino-4-trifluoromethyl-2-alkoxyquinoline. The synthesized compounds were characterized using NMR, light absorption, steady-state and time-resolved fluorescent spectroscopy. Some of the compounds displayed high brightness with Tb3+, Eu3+, and Dy3+. Obtained reactive lanthanide chelates can be easily attached to biological molecules. The probes demonstrated high performance in molecular beaconbased DNA hybridization assays (sub-pico molar detection limit), in bacterial proteome labeling, and in live cell imaging.

Citation Download Citation

Shyamala Pillai, Laura Wirpsza, Maxim Kozlov, Salvatore A. E Marras, Lev N. Krasnoperov, and Arkady Mustaev "New cross-linking quinoline- and quinolone-based luminescent lanthanide probes for sensitive labeling", Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82331C (2 February 2012); https://doi.org/10.1117/12.921056

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