Wide-field and high-resolution optical imaging for early detection of oral neoplasia

Mark C. Pierce; Richard A. Schwarz; Kelsey Rosbach; Darren Roblyer; Tim Muldoon; Michelle D. Williams; Adel K. El-Naggar; Ann M. Gillenwater; Rebecca Richards-Kortum

doi:10.1117/12.848347

2 March 2010 Wide-field and high-resolution optical imaging for early detection of oral neoplasia

Mark C. Pierce, Richard A. Schwarz, Kelsey Rosbach, Darren Roblyer, Tim Muldoon, Michelle D. Williams, Adel K. El-Naggar, Ann M. Gillenwater, Rebecca Richards-Kortum

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Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 75481U (2010) https://doi.org/10.1117/12.848347
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Current procedures for oral cancer screening typically involve visual inspection of the entire tissue surface at risk under white light illumination. However, pre-cancerous lesions can be difficult to distinguish from many benign conditions when viewed under these conditions. We have developed wide-field (macroscopic) imaging system which additionally images in cross-polarized white light, narrowband reflectance, and fluorescence imaging modes to reduce specular glare, enhance vascular contrast, and detect disease-related alterations in tissue autofluorescence. We have also developed a portable system to enable high-resolution (microscopic) evaluation of cellular features within the oral mucosa in situ. This system is a wide-field epi-fluorescence microscope coupled to a 1 mm diameter, flexible fiber-optic imaging bundle. Proflavine solution was used to specifically label cell nuclei, enabling the characteristic differences in N/C ratio and nuclear distribution between normal, dysplastic, and cancerous oral mucosa to be quantified. This paper discusses the technical design and performance characteristics of these complementary imaging systems. We will also present data from ongoing clinical studies aimed at evaluating diagnostic performance of these systems for detection of oral neoplasia.

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Mark C. Pierce, Richard A. Schwarz, Kelsey Rosbach, Darren Roblyer, Tim Muldoon, Michelle D. Williams, Adel K. El-Naggar, Ann M. Gillenwater, and Rebecca Richards-Kortum "Wide-field and high-resolution optical imaging for early detection of oral neoplasia", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75481U (2 March 2010); https://doi.org/10.1117/12.848347

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KEYWORDS

Imaging systems

Cancer

Optical imaging

Tissue optics

Luminescence

Diagnostics

In vivo imaging

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