Toward new fluorescent bioinspired sensors: interaction of poly(fluorene-phenylene) with phospholipid bilayers

R. Mallavia; F. J. Paya; A. Salinas; A. Estepa; C. R. Mateo

doi:10.1117/12.721505

22 May 2007 Toward new fluorescent bioinspired sensors: interaction of poly(fluorene-phenylene) with phospholipid bilayers

R. Mallavia, F. J. Paya, A. Salinas, A. Estepa, C. R. Mateo

Author Affiliations +

Proceedings Volume 6592, Bioengineered and Bioinspired Systems III; 659213 (2007) https://doi.org/10.1117/12.721505
Event: Microtechnologies for the New Millennium, 2007, Maspalomas, Gran Canaria, Spain

Abstract

Most of the conjugated polymer employed as fluorescent biosensors present low solubility and emission in aqueous environment. In order to solve this feature, we have reconstituted, in buffer phosphate, a neutral conjugated poly[9,9- bis(6'-bromohexil)-2,7-fluorene-co-alt-1,4-phenylene], as PFPBr₂ (insoluble in water), in the presence of an artificial zwitterionic phospholipids bilayers, as 1,2-dimyristoyl-sn-glycero-3-phospho-choline (DMPC). Quantum yield of PFPBr₂-DMPC was around 20% in phosphate buffer, it was identical value calculated from ammonium polyelectrolytes (PFPNMe³⁺). In addition, the maximum of bluish emission for buffer solution of PFPBr₂-DMPC was at 420nm, a red-shift emission with regard to chloroform solution (at 410 nm). The structural study at different concentrations of PFPBr₂ and DMPC was carried out using different approaches: steady state fluorescence spectroscopy, confocal fluorescence microscopy and calorimetry. A positive interaction takes place involving neutral conjugated polymer and zwitterionic phospholipids bilayer. Novels complexes or associations of poly(fluorene-phenylene) (PFPBr₂) and zwitterionic phospholipids (DMPC) have been suggested and visualized by epifluorescence. Phase transitions of the liposomes have been also detected by differential scanning calorimetry.

Citation Download Citation

R. Mallavia, F. J. Paya, A. Salinas, A. Estepa, and C. R. Mateo "Toward new fluorescent bioinspired sensors: interaction of poly(fluorene-phenylene) with phospholipid bilayers", Proc. SPIE 6592, Bioengineered and Bioinspired Systems III, 659213 (22 May 2007); https://doi.org/10.1117/12.721505

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