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30 May 2022 Rolling circle amplification tailored for plasmonic biosensors
Katharina Schmidt, Bernadette Lechner, Simone Hageneder, Yasaman Ahmadi, Maria Minunni, Erik Reimhult, Ivan Barišic, Jakub Dostálek
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Proceedings Volume PC12145, Biophotonics in Point-of-Care II; PC1214508 (2022) https://doi.org/10.1117/12.2621563
Event: SPIE Photonics Europe, 2022, Strasbourg, France
Abstract
Rolling circle amplification (RCA) for enhancement of the sensor response utilizing surface plasmon resonance and surface plasmon-enhanced fluorescence spectroscopy is reported. In order to maximize the efficiency of RCA, dedicated biointerface is developed for specific capture of target analyte and to guide the generated long oligonucleotide chains at the sensor surface within the confined probing surface plasmon field. The enhancement of the limit of detection by two orders of magnitude to 260 fM concentration is achieved for molecular ensembles and possible counting of individual captured molecules enables reaching limit of detection at low fM concentrations.
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Katharina Schmidt, Bernadette Lechner, Simone Hageneder, Yasaman Ahmadi, Maria Minunni, Erik Reimhult, Ivan Barišic, and Jakub Dostálek "Rolling circle amplification tailored for plasmonic biosensors", Proc. SPIE PC12145, Biophotonics in Point-of-Care II, PC1214508 (30 May 2022); https://doi.org/10.1117/12.2621563
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KEYWORDS
Biosensors
Plasmonics
Sensors
Luminescence
Surface plasmons
Biological research
Microscopy
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