Study of the influence of laser of low potency to label red blood cells and plasmatic protein with <sup>99m</sup>Tc <i>in vitro</i>

Ricardo Q. Rolim; Elaine F.N.B. Carvalho; Edgar V. Nascimento; Grace M.L. Souza; Simey S.L.P. Magnata; Mario Bernardo-Filho; Maria T.J.A. Catanho

doi:10.1117/12.584319

10 September 2004 Study of the influence of laser of low potency to label red blood cells and plasmatic protein with ^99mTc in vitro

Ricardo Q. Rolim, Elaine F.N.B. Carvalho, Edgar V. Nascimento, Grace M.L. Souza, Simey S.L.P. Magnata, Mario Bernardo-Filho, Maria T.J.A. Catanho

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Proceedings Volume 5610, Laser Florence 2003: A Window on the Laser Medicine World; (2004) https://doi.org/10.1117/12.584319
Event: Laser Florence 2003: A Window on the Laser Medicine World, 2003, Florence, Italy

Abstract

This work aims to verify the effect of laser of low potency to label red blood cells and plasmatic protein with ^99mTc in vitro. The experiments were carried out by incubating of anticoagulant whole blood. Differents doses of gallium arsenide laser with energy density of 3j/cm², 6j/cm², 9 j/cm², 18 j/cm² and 804nm wavelength was applied. A stannous chloride solution of 1,2μg/mL was added the incubation for 60 minutes. After this the ^99mTc was added and the incubation was continued for another 10 minutes. Those were centrifuged, precipitated with thrichoroacetic acid 5% and mensured in a counter. The results shows that there is a significant decrease in the fixation of ^99mTc in red blood cells when the concentration of 3j/ cm² and (from 93 to 32.2%) and in occurs an increase %ATI from 12 to 54,6% of plasma protein. The laser promote modification on some properties of the red blood cellular membrane, probably, due to the metabolization of cellular that could be capable to generation the active metabolites.

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Ricardo Q. Rolim, Elaine F.N.B. Carvalho, Edgar V. Nascimento, Grace M.L. Souza, Simey S.L.P. Magnata, Mario Bernardo-Filho, and Maria T.J.A. Catanho "Study of the influence of laser of low potency to label red blood cells and plasmatic protein with ^99mTc in vitro", Proc. SPIE 5610, Laser Florence 2003: A Window on the Laser Medicine World, (10 September 2004); https://doi.org/10.1117/12.584319

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KEYWORDS

Blood

Plasma

Proteins

Technetium

In vitro testing

Gallium arsenide lasers

Ions

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