Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence

Peng Zhang; Shi-You Ding; Qi Xu; Steve Smith; Garry Rumbles; Michael E. Himmel

doi:10.1117/12.560231

14 October 2004 Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence

Peng Zhang, Shi-You Ding, Qi Xu, Steve Smith, Garry Rumbles, Michael E. Himmel

Proceedings Volume 5513, Physical Chemistry of Interfaces and Nanomaterials III; (2004) https://doi.org/10.1117/12.560231
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to self-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the 6x-histidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.

Citation Download Citation

Peng Zhang, Shi-You Ding, Qi Xu, Steve Smith, Garry Rumbles, and Michael E. Himmel "Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence", Proc. SPIE 5513, Physical Chemistry of Interfaces and Nanomaterials III, (14 October 2004); https://doi.org/10.1117/12.560231

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