Toward Fourier interferometry fluorescence excitation/emission imaging of malignant cells combined with photoacoustic microscopy

Elli Kohen; Joseph G. Hirschberg; John P. Berry; Nuri Ozkutuk; Ceren Ornek; Marco Monti; Roger M. Leblanc; Dietrich O. Schachtschabel; Sumaira Haroon

doi:10.1117/12.500999

14 October 2003 Toward Fourier interferometry fluorescence excitation/emission imaging of malignant cells combined with photoacoustic microscopy

Elli Kohen, Joseph G. Hirschberg, John P. Berry, Nuri Ozkutuk, Ceren Ornek, Marco Monti, Roger M. Leblanc, Dietrich O. Schachtschabel, Sumaira Haroon

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Proceedings Volume 5143, Novel Optical Instrumentation for Biomedical Applications; (2003) https://doi.org/10.1117/12.500999
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Dual excitation fluorescence imaging has been used as a first step towards multi-wavelength excitation/emission fluorescence spectral imaging. Target cells are transformed keratinocytes, and other osteosarcoma, human breast and color cancer cells. Mitochondrial membrane potential probes, e.g. TMRM (tetramethylrhodamine methyl ester), Mitotracker Green (Molecular Probes, Inc., Eugene OR,USA) and a recently synthesized mitochondrial oxygen probe, [PRE,P1"- pyrene butyl)-2-rhodamine ester] allow dual excitation in the UV plus in teh blue-green spectral regions. Also, using the natural endogenous probe NAD(P)H, preliminary results indicate mitochondrial responses to metabolic challenges (e.g. glucose addition), plus changes in mitochonrial distribution and morphology. In terms of application to biomedicine (for diagnostiscs, prognostsics and drug trials) three parameters have been selected in addition to the natural probe NAD(P)H, i.e. vital fluorescence probing of mitochondria, lysosomes and Golgi apparatus. It is hoped that such a multiparameter approach will allow malignant cell characterization and grading. A new area being introduced is the use of similar methodology for biotechnical applications such as the study of the hydrogen-producing alga Chlamydomonas Reinhardtii, and possible agricultural applications, such as Saccharomyces yeast for oenology. Complementation by Photoacoustic Microscopy is also contemplated, to study the internal conversion component which follows the excitation by photons.

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Elli Kohen, Joseph G. Hirschberg, John P. Berry, Nuri Ozkutuk, Ceren Ornek, Marco Monti, Roger M. Leblanc, Dietrich O. Schachtschabel, and Sumaira Haroon "Toward Fourier interferometry fluorescence excitation/emission imaging of malignant cells combined with photoacoustic microscopy", Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); https://doi.org/10.1117/12.500999

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KEYWORDS

Luminescence

Glucose

Photoacoustic microscopy

Interferometry

Biomedical optics

Colorectal cancer

Imaging spectroscopy

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