Nad(P)H vs. Schiff base fluorescence by spectroscopy, imaging, and maximum sensitivity micrographs at the convergence of cellular detoxification, senescence, and transformation

Elli Kohen; Joseph G. Hirschberg; Cahide Kohen; Marco Monti

doi:10.1117/12.347520

3 May 1999 Nad(P)H vs. Schiff base fluorescence by spectroscopy, imaging, and maximum sensitivity micrographs at the convergence of cellular detoxification, senescence, and transformation

Elli Kohen, Joseph G. Hirschberg, Cahide Kohen, Marco Monti

Author Affiliations +

Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV; (1999) https://doi.org/10.1117/12.347520
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Two intracellular fluorochromes, NAD(P)H and Schiff Bases, provide monitoring of energy metabolism and photoperoxidations. Fluorochrome spectra and topographic distribution are measured in a microspectrofluorometer, pixel by pixel using a CCD. The mitochondrial arrangement of Saccharomyces cerevisie and metabolic activity at nuclear kidney epithelial sites is revealed. A kind of accelerated photoaging results in the accumulation of Schiff pigment. Schiff base emission is red-shifted, and it may be preceded by photo-oxidation of NAD(P)H. UVA production of oxygen radicals and peroxides may influence detoxification, senescence and/or transformation. Besides lysosomes, mitochondrial energy metabolism and ER and Golgi detoxification are open to study as multi-organelle complexes with fluorescent xenobiotics and probes. Melanocytes vs. melanoma cells in culture will be investigated using a new compact interferometer for Fourier coding of both emission and excitation spectra. Surprisingly, the photographic method, using the highest sensitivity films, may sometimes produce excellent structural detail. However, for kinetic studies, the CCD, or equivalent, is required. There is good potential for applications in diagnostics and prognostics plus the evaluation of new biopharmeceuticals.

Citation Download Citation

Elli Kohen, Joseph G. Hirschberg, Cahide Kohen, and Marco Monti "Nad(P)H vs. Schiff base fluorescence by spectroscopy, imaging, and maximum sensitivity micrographs at the convergence of cellular detoxification, senescence, and transformation", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); https://doi.org/10.1117/12.347520

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