Paper
29 July 2003 Validation of NIRS in measuring tissue hemoglobin concentration and oxygen saturation on ex vivo and isolated limb models
Xiaorong Xu, Wen Zhu, Vikram Padival, Mengna Xia, Xuefeng Cheng, Robin Bush, Linda Christenson, Tim Chan, Tim Doherty, Angelo Iatridis
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Abstract
Photonify’s tissue spectrometer uses Near-Infrared Spectroscopy for real-time, noninvasive measurement of hemoglobin concentration and oxygen saturation [SO2] of biological tissues. The technology was validated by a series of ex vivo and animal studies. In the ex vivo experiment, a close loop blood circulation system was built, precisely controlling the oxygen saturation and the hemoglobin concentration of a liquid phantom. Photonify’s tissue spectrometer was placed on the surface of the liquid phantom for real time measurement and compared with a gas analyzer, considered the gold standard to measure oxygen saturation and hemoglobin concentration. In the animal experiment, the right hind limb of each dog accepted onto the study was surgically removed. The limb was kept viable by connecting the femoral vein and artery to a blood-primed extracorporeal circuit. Different concentrations of hemoglobin were obtained by adding designated amount of saline solution into the perfusion circuit. Photonify’s tissue spectrometers measured oxygen saturation and hemoglobin concentration at various locations on the limb and compared with gas analyzer results. The test results demonstrated that Photonify’s tissue spectrometers were able to detect the relative changes in tissue oxygen saturation and hemoglobin concentration with a high linear correlation compared to the gas analyzer
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Xiaorong Xu, Wen Zhu, Vikram Padival, Mengna Xia, Xuefeng Cheng, Robin Bush, Linda Christenson, Tim Chan, Tim Doherty, and Angelo Iatridis "Validation of NIRS in measuring tissue hemoglobin concentration and oxygen saturation on ex vivo and isolated limb models", Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); https://doi.org/10.1117/12.476562
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Cited by 10 scholarly publications.
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KEYWORDS
Tissue optics

Oxygen

Spectroscopy

Blood

Tissues

Calibration

Near infrared spectroscopy

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