Near-infrared Raman spectroscopy to detect anti-Toxoplasma gondii antibodies in blood sera of domestic cats

Janaina Duarte; Marcos T. T. Pacheco; Landulfo Silveira Jr.; Rosangela Zacarias Machado; Rodrigo A.B. Lopes Martins; Renato Amaro Zangaro; Antonio G. J. Balbin Villaverde

doi:10.1117/12.427767

21 May 2001 Near-infrared Raman spectroscopy to detect anti-Toxoplasma gondii antibodies in blood sera of domestic cats

Janaina Duarte, Marcos T. T. Pacheco, Landulfo Silveira Jr., Rosangela Zacarias Machado, Rodrigo A.B. Lopes Martins, Renato Amaro Zangaro, Antonio G. J. Balbin Villaverde

Author Affiliations +

Proceedings Volume 4244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI; (2001) https://doi.org/10.1117/12.427767
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Near-infrared (NIR) Raman spectroscopy has been studied for the last years for many biomedical applications. It is a powerful tool for biological materials analysis. Toxoplasmosis is an important zoonosis in public health, cats being the principal responsible for the transmission of the disease in Brazil. The objective of this work is to investigate a new method of diagnosis of this disease. NIR Raman spectroscopy was used to detect anti Toxoplasma gondii antibodies in blood sera from domestic cats, without sample preparation. In all, six blood serum samples were used for this study. A previous serological test was done by the Indirect Immunoenzymatic Assay (ELISA) to permit a comparative study between both techniques and it showed that three serum samples were positive and the other three were negative to toxoplasmosis. Raman spectra were taken for all the samples and analyzed by using the principal components analysis (PCA). A diagnosis parameter was defined from the analysis of the second and third principal components of the Raman spectra. It was found that this parameter can detect the infection level of the animal. The results have indicated that NIR Raman spectroscopy, associated to the PCA can be a promising technique for serological analysis, such as toxoplasmosis, allowing a fast and sensitive method of diagnosis.

Citation Download Citation

Janaina Duarte, Marcos T. T. Pacheco, Landulfo Silveira Jr., Rosangela Zacarias Machado, Rodrigo A.B. Lopes Martins, Renato Amaro Zangaro, and Antonio G. J. Balbin Villaverde "Near-infrared Raman spectroscopy to detect anti-Toxoplasma gondii antibodies in blood sera of domestic cats", Proc. SPIE 4244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI, (21 May 2001); https://doi.org/10.1117/12.427767

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