IR-Spectroscopic imaging applied to human colon biopsy

Ingo H. Gersonde; Martina Meinke; Y. Becker; Uwe Bindig; Bernd Eppich; G. Mueller

doi:10.1117/12.379586

14 March 2000 IR-Spectroscopic imaging applied to human colon biopsy

Ingo H. Gersonde, Martina Meinke, Y. Becker, Uwe Bindig, Bernd Eppich, G. Mueller

Proceedings Volume 3920, Spectral Imaging: Instrumentation, Applications, and Analysis; (2000) https://doi.org/10.1117/12.379586
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Tissue samples from colon biopsy have been analyzed using FT-IR microscopy. For differentiation of cancerous versus normal tissue and for imaging of tissue structure linear and quadratic discriminant analysis was applied. A large number of samples has been used for statistical parametrization. After IR mapping, the samples have been analyzed with common pathological techniques. Comparing the pathological classification with the results of the discriminant analysis the resolving power of the later is estimated. The influence of filtering (using derivative spectra, baseline correction, and normalization) has been investigated. In vivo application of IR-spectroscopy using IR laser diodes or grating spectrometers for fast measurements will lead to a reduction of data concerning spectral range and resolution. The accompanying loss in differentiation of tissue is discussed. Images constructed with the discriminant analysis are compared to corresponding images from visible microscopy.

Citation Download Citation

Ingo H. Gersonde, Martina Meinke, Y. Becker, Uwe Bindig, Bernd Eppich, and G. Mueller "IR-Spectroscopic imaging applied to human colon biopsy", Proc. SPIE 3920, Spectral Imaging: Instrumentation, Applications, and Analysis, (14 March 2000); https://doi.org/10.1117/12.379586

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Tissues

Statistical analysis

Biopsy

Error analysis

Data analysis

Tumors

Visible radiation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years