Visible spectroscopic imaging studies of normal and ischemic dermal tissue

Karel J. Zuzak; Michael D. Schaeberle; E. Neil Lewis; Ira W. Levin

doi:10.1117/12.384958

8 May 2000 Visible spectroscopic imaging studies of normal and ischemic dermal tissue

Karel J. Zuzak, Michael D. Schaeberle, E. Neil Lewis, Ira W. Levin

Proceedings Volume 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques; (2000) https://doi.org/10.1117/12.384958
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We describe a non-invasive, in vivo hyperspectral imaging method for visualizing the spatial distribution of dermal tissue oxygenation. Real-time images of the dermis are acquired both at multiple, contiguous wavelengths and at relatively narrow spectral bandwidths to generate a data cube consisting of one spectral and two spatial dimensions. For data collection, the sample area is illuminated by radiation, which is delivered by liquid light guides from a quartz tungsten halogen source. Reflected light from the sample is first passed through a liquid crystal tunable filter and then imaged onto a silicon charged coupled device detector. The subsequently digitized data are presented in terms of spectral images reflecting multivariate least squares analyses based upon linear combinations of oxy- and deoxyhemoglobin reference spectra. The generated gray scale images directly represent the varying spatial distributions of dermal tissue oxygenation. As an example, imaging data are obtained from normal tissue and induced ischemic tissue for which both the venous and arterial blood flow was artificially occluded.

Citation Download Citation

Karel J. Zuzak, Michael D. Schaeberle, E. Neil Lewis, and Ira W. Levin "Visible spectroscopic imaging studies of normal and ischemic dermal tissue", Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); https://doi.org/10.1117/12.384958

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