D-light for laparoscopic fluorescence diagnosis

Johannes Gahlen; Hans-Heinrich Laubach; Josef Stern; Jochen Pressmar; Mathias Pietschmann; Christian Herfarth

doi:10.1117/12.351509

6 July 1999 D-light for laparoscopic fluorescence diagnosis

Johannes Gahlen, Hans-Heinrich Laubach, Josef Stern, Jochen Pressmar, Mathias Pietschmann, Christian Herfarth

Proceedings Volume 3592, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VIII; (1999) https://doi.org/10.1117/12.351509
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

To evaluate the role of ALA induced fluorescence diagnosis in laparoscopic surgery, we induced peritoneal carcinosis in rats by multilocular intraabdominal tumorcell implantation (CC531). The animals were photosensitized by intraabdominal ALA lavage. Laparoscopy was performed with both, conventional white and then blue light (D-Light, KARL STORZ Germany) excitation. Laparoscopy with conventional white light showed peritoneal carcinoma foci from 0.1 to 2 cm in diameter. All macroscopically visible tumors (n equals 142) were fluorescence positive after laparoscopic blue light excitation. In addition, 30 laparoscopic not visible (white light) tumors showed fluorescence and were histologically confirmed as colon carcinoma metastases. We conclude that only ALA induced laparoscopic fluorescence detection after blue light excitation is the adequate method to detect the entire extent of the intraabdominal tumor spread. Fluorescence laparoscopy is essential for laparoscopic staging of colorectal cancer because of a higher rate of cancer foci detection.

Citation Download Citation

Johannes Gahlen, Hans-Heinrich Laubach, Josef Stern, Jochen Pressmar, Mathias Pietschmann, and Christian Herfarth "D-light for laparoscopic fluorescence diagnosis", Proc. SPIE 3592, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VIII, (6 July 1999); https://doi.org/10.1117/12.351509

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