Two-dimensional fluorescence spectroscopy for application in biotechnology

Carsten Lindemann; S. Marose; Thomas-Helmut Scheper; Hans Ole Nielsen; Bernd Hitzmann; K.-H. Belgardt

doi:10.1117/12.338986

10 February 1999 Two-dimensional fluorescence spectroscopy for application in biotechnology

Carsten Lindemann, S. Marose, Thomas-Helmut Scheper, Hans Ole Nielsen, Bernd Hitzmann, K.-H. Belgardt

Proceedings Volume 3534, Environmental Monitoring and Remediation Technologies; (1999) https://doi.org/10.1117/12.338986
Event: Photonics East (ISAM, VVDC, IEMB), 1998, Boston, MA, United States

Abstract

A wide range of excitation and emission wavelengths is measured using the technique of two-dimensional (2D-) fluorescence spectroscopy. In a single, so called, two- dimensional fluorescence spectrum several biogenic fluorophors like proteins, vitamins and coenzymes can be detected simultaneously. This can give important information for bioprocess monitoring and control. An optical sensor (BioView^R) for on line fluorescence measurements at industrial (bio)-processes was used to get the results presented in this paper. This BioView^R-sensor is optimized to work in the harsh environment of production sites in biotechnological industry and -- using an optical light guide system with open-end detection -- it is very well suited for in vivo measurements, because it is non-invasive and the on line data can be performed in-situ.

Citation Download Citation

Carsten Lindemann, S. Marose, Thomas-Helmut Scheper, Hans Ole Nielsen, Bernd Hitzmann, and K.-H. Belgardt "Two-dimensional fluorescence spectroscopy for application in biotechnology", Proc. SPIE 3534, Environmental Monitoring and Remediation Technologies, (10 February 1999); https://doi.org/10.1117/12.338986

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