Application of fluorescence spectroscopy for on-line bioprocess monitoring and control

Daniela Boehl; D. Solle; Hans Josef Toussaint; M. Menge; G. Renemann; Carsten Lindemann; Bernd Hitzmann; Thomas-Helmut Scheper

doi:10.1117/12.417395

13 February 2001 Application of fluorescence spectroscopy for on-line bioprocess monitoring and control

Daniela Boehl, D. Solle, Hans Josef Toussaint, M. Menge, G. Renemann, Carsten Lindemann, Bernd Hitzmann, Thomas-Helmut Scheper

Author Affiliations +

Proceedings Volume 4201, Optical Methods for Industrial Processes; (2001) https://doi.org/10.1117/12.417395
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States

Abstract

12 Modern bioprocess control requires fast data acquisition and in-time evaluation of bioprocess variables. On-line fluorescence spectroscopy for data acquisition and the use of chemometric methods accomplish these requirements. The presented investigations were performed with fluorescence spectrometers with wide ranges of excitation and emission wavelength. By detection of several biogenic fluorophors (amino acids, coenzymes and vitamins) a large amount of information about the state of the bioprocess are obtained. For the evaluation of the process variables partial least squares regression is used. This technique was applied to several bioprocesses: the production of ergotamine by Claviceps purpurea, the production of t-PA (tissue plasminogen activator) by animal cells and brewing processes. The main point of monitoring the brewing processes was to determine the process variables cell count and extract concentration.

Citation Download Citation

Daniela Boehl, D. Solle, Hans Josef Toussaint, M. Menge, G. Renemann, Carsten Lindemann, Bernd Hitzmann, and Thomas-Helmut Scheper "Application of fluorescence spectroscopy for on-line bioprocess monitoring and control", Proc. SPIE 4201, Optical Methods for Industrial Processes, (13 February 2001); https://doi.org/10.1117/12.417395

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