High-resolution whole field fluorescence lifetime imaging of fluorophore distribution and environment

Mark J. Dayel; Keith Dowling; Sam C. W. Hyde; Christopher Dainty; Paul M. W. French; P. Vourdas; M. John Lever; Anthony K. L. Dymoke-Bradshaw; Jonathan D. Hares; Paul A. Kellett

doi:10.1117/12.297925

13 January 1998 High-resolution whole field fluorescence lifetime imaging of fluorophore distribution and environment

Mark J. Dayel, Keith Dowling, Sam C. W. Hyde, Christopher Dainty, Paul M. W. French, P. Vourdas, M. John Lever, Anthony K. L. Dymoke-Bradshaw, Jonathan D. Hares, Paul A. Kellett

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Proceedings Volume 3196, Optical and Imaging Techniques for Biomonitoring III; (1998) https://doi.org/10.1117/12.297925
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

We report the development of a high temporal resolution fluorescence lifetime imaging (FLIM) system suing a time- gated image intensifier to provide whole field FLIM images. The gate width has been optimized to 110 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences of 20 ps, have been detected. Environmental changes of the fluorescent indicator, Lucifer Yellow, have been sensed by measuring changes in its fluorescence lifetime in the presence of the protein albumin. We also present provisional fluorescence lifetime images of tissue constituents.

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Mark J. Dayel, Keith Dowling, Sam C. W. Hyde, Christopher Dainty, Paul M. W. French, P. Vourdas, M. John Lever, Anthony K. L. Dymoke-Bradshaw, Jonathan D. Hares, and Paul A. Kellett "High-resolution whole field fluorescence lifetime imaging of fluorophore distribution and environment", Proc. SPIE 3196, Optical and Imaging Techniques for Biomonitoring III, (13 January 1998); https://doi.org/10.1117/12.297925

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KEYWORDS

Fluorescence lifetime imaging

Environmental sensing

Image intensifiers

Luminescence

Picosecond phenomena

Imaging systems

Proteins

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