Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier

Keith Dowling; Sam C. W. Hyde; Nicholas P. Barry; Christopher Dainty; Paul M. W. French; Alun J. Hughes; M. John Lever; Anthony K. L. Dymoke-Bradshaw; Jonathan D. Hares; Paul A. Kellett

doi:10.1117/12.273522

7 May 1997 Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier

Keith Dowling, Sam C. W. Hyde, Nicholas P. Barry, Christopher Dainty, Paul M. W. French, Alun J. Hughes, M. John Lever, Anthony K. L. Dymoke-Bradshaw, Jonathan D. Hares, Paul A. Kellett

Author Affiliations +

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273522
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We report the demonstration of a high temporal resolution fluorescence lifetime imaging (FLIM) system using a time- gated image intensifier to provide whole field FLIM images. The gate width has been optimized to 110 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences of 20 ps, have been detected. Environmental changes of the fluorescent indicator, Lucifer Yellow, have been sensed by measuring changes in its fluorescence lifetime when unbound and when bound to the protein albumin.

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Keith Dowling, Sam C. W. Hyde, Nicholas P. Barry, Christopher Dainty, Paul M. W. French, Alun J. Hughes, M. John Lever, Anthony K. L. Dymoke-Bradshaw, Jonathan D. Hares, and Paul A. Kellett "Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273522

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