Genotoxity of pyrolysis products originating from laser-treated porcine tissues

Bernd Stocker; Ulla Plappert; Lothar W. Weber; Theodor M. Fliedner; Bernd Treffler; Thomas H. Meier

doi:10.1117/12.229567

10 January 1996 Genotoxity of pyrolysis products originating from laser-treated porcine tissues

Bernd Stocker, Ulla Plappert, Lothar W. Weber, Theodor M. Fliedner, Bernd Treffler, Thomas H. Meier

Proceedings Volume 2624, Laser-Tissue Interaction and Tissue Optics; (1996) https://doi.org/10.1117/12.229567
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

Different types of porcine tissue were irradiated with a surgical CO₂ laser. The generated aerosols were sampled on glass fiber filters and incubated with human peripheral blood cells. Afterwards, these exposed cells were subjected to the comet assay. The single cell gel electrophoresis or comet assay represents a powerful technique for the detection of DNA strand breaks in eukaryotic cells. In short, the electrophoretic mobility of DNA fragments is proportional to the quantity of DNA damage caused by the genotoxic agent in question. By investigating porcine tissue laser pyrolysis products, it is demonstrated that the comet assay is an appropriate tool to assess the genotoxic capacity of even a heterogeneously composed class of substances with unknown modes of action and interaction.

Citation Download Citation

Bernd Stocker, Ulla Plappert, Lothar W. Weber, Theodor M. Fliedner, Bernd Treffler, and Thomas H. Meier "Genotoxity of pyrolysis products originating from laser-treated porcine tissues", Proc. SPIE 2624, Laser-Tissue Interaction and Tissue Optics, (10 January 1996); https://doi.org/10.1117/12.229567

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