Immunologic methods for monitoring carcinogen exposure

Regina M. Santella; Frederica P. Perera; Yu Jing Zhang; Chen Jen Chen; Tie Lan Young

doi:10.1117/12.140259

9 March 1993 Immunologic methods for monitoring carcinogen exposure

Regina M. Santella, Frederica P. Perera, Yu Jing Zhang, Chen Jen Chen, Tie Lan Young

Proceedings Volume 1716, International Conference on Monitoring of Toxic Chemicals and Biomarkers; (1993) https://doi.org/10.1117/12.140259
Event: Environmental Sensing '92, 1992, Berlin, Germany

Abstract

Immunologic methods have been developed for monitoring human exposure to environmental and occupational carcinogens. These methods involve the development of monoclonal and polyclonal antisera which specifically recognize the carcinogens themselves or their DNA or protein adducts. Antisera recognizing the DNA adducts of several polycyclic aromatic hydrocarbon diol epoxides have been used in competitive enzyme-linked immunosorbent assays to monitor adducts in tissue or blood samples. Elevated levels of DNA adducts have been seen in mononuclear cells of smokers and in total white blood cells of foundry and coke oven workers. Environmental exposure to PAH has been measured in individuals living in a highly polluted region of Poland. Antisera recognizing PAH-DNA adducts have also been used in immunohistochemical studies to monitor adducts in specific cells of biopsy samples. The DNA adducts of aflatoxin B₁ have been monitored in liver tissue of hepatocellular carcinoma patients in Taiwan. Detectable adducts were seen in 50 - 70% of the patients suggesting that dietary exposure to this carcinogen may be a risk factor for cancer induction. Thus, immunoassays for monitoring exposure to carcinogens are an important tool in epidemiologic studies.

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Regina M. Santella, Frederica P. Perera, Yu Jing Zhang, Chen Jen Chen, and Tie Lan Young "Immunologic methods for monitoring carcinogen exposure", Proc. SPIE 1716, International Conference on Monitoring of Toxic Chemicals and Biomarkers, (9 March 1993); https://doi.org/10.1117/12.140259

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KEYWORDS

Blood

Tissues

Control systems

Proteins

Cancer

Liver

Biopsy

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